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Seawater transfer down-regulates C-type natriuretic peptide-3 expression in prolactin-producing cells of Japanese eel: Negative correlation with plasma chloride concentration.
Molecular and Cellular Endocrinology ( IF 3.8 ) Pub Date : 2020-03-03 , DOI: 10.1016/j.mce.2020.110780
Yukitoshi Katayama 1 , Marty Kwok-Shing Wong 2 , Makoto Kusakabe 3 , Megumi Fujio 1 , Natsuki Takahashi 1 , Miku Yaguchi 1 , Takehiro Tsukada 1
Affiliation  

In euryhaline fishes, atrial and B-type natriuretic peptides are important hormones in hypo-osmoregulation, whereas osmoregulatory functions of C-type natriuretic peptides (CNPs) remain to be investigated. Although four CNP isoforms (CNP1-4) are mainly expressed in the brain, multiorgan expression of CNP3 was found in euryhaline Japanese eel, Anguilla japonica. Here we identified the CNP3-expressing cells and examined their response to osmotic stress in eel. CNP3 was expressed in several endocrine cells: prolactin-producing cells (pituitary), glucagon-producing cells (pancreas), and cardiomyocytes (heart). Pituitary CNP3 expression was the highest among organs and was decreased following seawater transfer, followed by a decrease in the freshwater-adaptating (hyper-osmoregulatory) hormone prolactin. We also showed the negative correlation between CNP3/prolactin expression in the pituitary and plasma Cl concentration, but not for plasma Na+ concentration. These results suggest that CNP3 in the pituitary (and pancreas) plays a critical role in freshwater adaptation of euryhaline eel together with prolactin.



中文翻译:

海水转移下调了日本鳗催乳素产生细胞中C型利钠肽3的表达:与血浆氯化物浓度呈负相关。

在鱼腥鱼中,心房和B型利尿钠肽是低渗调节中的重要激素,而C型利尿钠肽(CNP)的渗透调节功能仍有待研究。虽然4个CNP亚型(CNP1-4)主要表达在脑,CNP3的多器官中表达广盐日本鳗鲡发现,鳗鲡。在这里,我们鉴定了CNP3表达细胞,并检查了它们对鳗鱼渗透压的反应。CNP3在几种内分泌细胞中表达:催乳素产生细胞(垂体),胰高血糖素产生细胞(胰腺)和心肌细胞(心脏)。垂体CNP3表达在各器官中最高,并在海水转移后降低,其后淡水适应(高渗透调节)激素催乳素减少。我们还发现在垂体和血浆氯CNP3 /催乳素表达的负相关-浓度,但不用于血浆的Na +浓度。这些结果表明,垂体(和胰腺)中的CNP3在淡水鳗鱼鳗鱼和催乳素的适应中起着关键作用。

更新日期:2020-03-03
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