Ovarian development is a complex process controlled by precise coordination of multiple factors. The targeted gene knockout technique is a powerful tool to study the functions of these factors. The successful application of this technique in mice in the past three decades has significantly enhanced our understanding on the molecular mechanism of ovarian development. Recently, with the advent of genome editing techniques, targeted gene knockout research can be carried out in many species. Zebrafish has emerged as an excellent model system to study the control of ovarian development. Dozens of genes related to ovarian development have been knocked out in zebrafish in recent years. Much new information and perspectives on the molecular mechanism of ovarian development have been obtained from these mutant zebrafish. Some findings have challenged conventional views. Several genes have been identified for the first time in vertebrates to control ovarian development. Focusing on ovarian development, the purpose of this review is to briefly summarize recent findings using these gene knockout zebrafish models, and compare these findings with mammalian models. These established mutants and rapid development of gene knockout techniques have prompted zebrafish as an ideal animal model for studying ovarian development.

卵巢发育是一个由多个因素精确协调控制的复杂过程。靶向基因敲除技术是研究这些因素功能的有力工具。在过去的三十年中，该技术在小鼠中的成功应用极大地增强了我们对卵巢发育的分子机制的了解。最近，随着基因组编辑技术的出现，可以在许多物种中进行靶向基因敲除研究。斑马鱼已经成为研究卵巢发育控制的优秀模型系统。近年来，数十种与卵巢发育有关的基因已在斑马鱼中被剔除。从这些突变斑马鱼获得了许多有关卵巢发育的分子机制的新信息和观点。一些发现挑战了传统观点。在脊椎动物中首次鉴定出几种基因来控制卵巢发育。着眼于卵巢发育，本文的目的是简要概述使用这些基因敲除斑马鱼模型的最新发现，并将这些发现与哺乳动物模型进行比较。这些确定的突变体和基因敲除技术的迅速发展促使斑马鱼成为研究卵巢发育的理想动物模型。