Bisphenol A diglycidyl ether (BADGE) is an epoxy resin used for the inner coating of canned food and beverages. BADGE can easily migrate from the containers and become a contaminant. In this study, we examined the effects of BADGE exposure to the dams on the behavioral, structural, and developmental abnormalities in the offspring. Female pregnant mice were fed with a diet containing BADGE (0.15 or 1.5 mg/kg/day) during gestation and lactation periods. In an open field test, the time spent in the corner area significantly increases in male mice of high-dose BADGE group at 5 weeks old. The histological analysis using offspring brain at postnatal day 1 delivered from BADGE (1.5 mg/kg/day)-treated dams demonstrates that positive signals of Forkhead box P2- and COUP-TF interacting protein 2 are restricted in each cortical layer, but not in the control brain. In addition, the maternal BADGE exposure reduces nestin-positive fibers of the radial glia and T-box transcription factor 2-positive intermediate progenitors in the inner subventricular zone. Furthermore, a direct BADGE exposure promotes neurite outgrowth and neuronal connection in the primary cultured cortical neurons. These data suggest that maternal BADGE exposure can accelerate neuronal differentiation in fetuses and induce anxiety-like behavior in juvenile mice.

双酚A二缩水甘油醚（BADGE）是用于罐装食品和饮料内涂层的环氧树脂。BADGE容易从容器中迁移出来并成为污染物。在这项研究中，我们检查了BADGE接触大坝对后代行为，结构和发育异常的影响。在妊娠和哺乳期，给雌性怀孕小鼠喂食含BADGE（0.15或1.5 mg / kg /天）的饮食。在开放式试验中，大剂量BADGE组的雄性小鼠在5周大时，在角区域花费的时间显着增加。使用BADGE（1.5 mg / kg / day）处理的水坝在出生后第1天使用后代大脑进行的组织学分析表明，在每个皮质层中均限制了Forkhead box P2-和COUP-TF相互作用蛋白2的阳性信号，但不在控制大脑中。此外，孕妇的BADGE暴露会减少心室内膜下区的放射状胶质的Nestin阳性纤维和T-box转录因子2阳性中间祖细胞。此外，直接BADGE暴露可促进原代培养的皮层神经元的神经突生长和神经元连接。这些数据表明母体BADGE暴露可以加速胎儿的神经元分化并诱导幼年小鼠的焦虑样行为。