Abstract The extraction of blueberry anthocyanins with natural deep eutectic solvents (NADES) based on choline chloride:glycerol:citric acid was optimized by simplex centroid design to find the best molar ratio for the solvent. Choline chloride:glycerol:citric acid at the molar ratio of 0.5:2:0.5 (NADES 6) was the optimal NADES for extraction. NADES 6 was as efficient as the conventional organic solvent (methanol:water:formic acid) when compared at the same sample:solvent ratio and yielded about 76 % of blueberry anthocyanins compared to an exhaustive extraction with the organic solvent. NADES 6 yielded higher proportion of arabinoside anthocyanins and lower proportion of galactoside anthocyanins than the organic solvent. Thus, NADES can be assumed as a cheaper substitute for organic solvents, yielding biocompatible extracts of blueberry that are selectively enriched in arabinoside anthocyanins.

中文翻译：

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天然低共熔溶剂作为提取蓝莓花青素的生物相容性工具

摘要 以氯化胆碱：甘油：柠檬酸为基础的天然低共熔溶剂（NADES）通过单纯形心设计优化蓝莓花青素的提取，寻找最佳溶剂摩尔比。摩尔比为 0.5:2:0.5 (NADES 6) 的氯化胆碱:甘油:柠檬酸是提取的最佳 NADES。NADES 6 与传统有机溶剂（甲醇：水：甲酸）一样有效，在相同的样品：溶剂比例下进行比较，与使用有机溶剂彻底提取相比，蓝莓花青素的产量约为 76%。与有机溶剂相比，NADES 6 产生更高比例的阿拉伯糖苷花色苷和更低比例的半乳糖苷花色苷。因此，可以假设 NADES 是一种更便宜的有机溶剂替代品，