Triazine herbicide atrazine is considered to be moderately toxic to various aquatic animals. The aim of our study was to evaluate the acute embryotoxicity of atrazine and its two degradation products, desisopropylatrazine and desethylatrazine, and their mixture to the early life stages of zebrafish (Danio rerio) by means of a modified method of the Fish Embryo Acute Toxicity (FET) Test – OECD guideline 236. Toxic effects were studied by the evaluation of lethal endpoints and development of disorders. Furthermore, sublethal endpoints such as hatching rate, formation of somites, development of eyes, spontaneous movement, heartbeat, blood circulation, pigmentation and occurrence of edema at 24, 48, 72 and 96 h post fertilization were assessed. Newly fertilized eggs were exposed to various concentrations of atrazine, desisopropylatrazine and desethylatrazine, and their combination 0.3, 30, 100, 300, 1000, 3,000 and 10,000 μg/l, which represent environmentally relevant levels of these pollutants in surface waters and multiples of these concentrations to find out if the toxic effect depends on dose. Single substances and their combination were not associated with a negative effect on mortality. Rare malformations were observed during these embryonal toxicity tests. Only pericardial edema was recorded during the monitored observation. A significant increase in the occurrence of pericardial edema between the control 0% and the experimental group 17.6 %) was found only in the group exposed to the highest concentration of a triazine herbicide combination (10,000 μg/l) at 72 and 96 h post fertilization. Obtained results indicate that especially higher not environmentally relevant concentrations of atrazine, its metabolites or their combination present a potencial risk of embryotoxicity for zebrafish.

三嗪除草剂at去津被认为对各种水生动物具有中等毒性。我们的研究目的是评估阿特拉津及其两种降解产物desisopropylatrazine和desethylatrazine及其混合物对斑马鱼生命早期的急性胚胎毒性（Danio rerio）通过鱼类胚胎急性毒性（FET）测试– OECD准则236的改进方法。通过评估致死终点和疾病发展来研究毒性。此外，评估了受精后24、48、72和96小时的亚致死终点，如孵化率，体节的形成，眼睛的发育，自发运动，心跳，血液循环，色素沉着和浮肿的发生。将新受精的卵暴露于不同浓度的阿特拉津，去异丙基阿特拉津和去乙基阿特拉津及其组合0.3、30、100、300、1000、3,000和10,000μg/ l，这代表了地表水中这些污染物的环境相关水平，并且是这些污染物的倍数来确定其毒性作用是否取决于剂量。单一物质及其组合对死亡率没有负面影响。在这些胚胎毒性试验中观察到罕见的畸形。在监测期间仅记录心包水肿。仅在受精后72 h和96 h暴露于最高浓度的三嗪除草剂组合（10,000μg/ l）的组中，对照组0％和实验组之间心包水肿的发生率显着增加（17.6％）。 。获得的结果表明，特别高的与环境无关的阿特拉津，其代谢产物或它们的组合存在潜在的斑马鱼胚胎毒性风险。在监测期间仅记录心包水肿。仅在受精后72 h和96 h暴露于最高浓度的三嗪除草剂组合（10,000μg/ l）的组中，对照组0％和实验组之间心包水肿的发生率显着增加（17.6％）。 。获得的结果表明，特别高的与环境无关的阿特拉津，其代谢产物或它们的组合存在潜在的斑马鱼胚胎毒性风险。在监测期间仅记录心包水肿。仅在受精后72 h和96 h暴露于最高浓度的三嗪除草剂组合（10,000μg/ l）的组中，对照组0％和实验组之间心包水肿的发生率显着增加（17.6％）。 。获得的结果表明，特别高的与环境无关的阿特拉津，其代谢产物或它们的组合存在潜在的斑马鱼胚胎毒性风险。