Several ecotoxicological studies assessed metal toxicity upon soil biota and other communities but were mainly focused on the study of a single chemical and usually under optimal conditions of temperature. Meanwhile an increasing global warming is leading to new scenarios by combining different stress factors; chemical stress and thermal stress. Presently, this study aims to assess the joint effects produced by cadmium and elevated temperature on earthworms different levels of biological complexity. Eisenia fetida earthworms were maintained at 19 °C and 26 °C and simultaneously exposed to four Cd concentrations (1.25, 2.5, 25 and 125 mg Cd/Kg soil) for 14 (Short term exposure) and 56 days (reproduction test). Endpoints were addressed at different levels of biological complexity: reproductive impairment (cocoons and juvenile productions), Cd tissue accumulation, mortality of adults, weight loss and cytotoxic effects (coelomocyte viability). In the Short term exposure, increase in temperature produced a larger accumulation of Cd. Hence, earthworms exposed to 125 mg Cd/kg soil under heat stress (26 °C) showed a two fold higher Cd accumulation comparing to those at 19 °C. Earthworms exposed to moderate-high concentrations of Cd (2.5–125 mg Cd/kg) and maintained at 26 °C showed severe weight loss and high mortality rates. The neutral red uptake capacity of coelomocytes extruded from earthworms exposed to the highest Cd concentration decreased after 14 d at 19 °C, and more markedly at 26 °C. The reproduction impairment (decreased number of cocoons) was enhanced after exposure to concentrations higher than 2.5 mg Cd/kg at 26 °C, and after exposure to 125 mg Cd/kg at 19 °C. Earthworm reproduction capability is highly vulnerable to the effect of toxicants at elevated temperatures and sublethal concentrations.

几项生态毒理学研究评估了金属对土壤生物群和其他群落的毒性，但主要集中在单一化学物质的研究上，并且通常在最佳温度条件下进行。同时，全球变暖的加剧通过结合不同的压力因素导致了新的情况。化学应力和热应力。目前，这项研究旨在评估镉和高温对earth不同程度的生物复杂性产生的联合影响。etwere保持在19°C和26°C，同时暴露于4种Cd浓度（1.25、2.5、25和125 mg Cd / Kg土壤）中暴露14天（短期暴露）和56天（繁殖试验）。在生物复杂性的不同水平上解决了终点问题：生殖功能障碍（茧和幼体），镉组织积累，成年死亡率，体重减轻和细胞毒性作用（白细胞活力）。在短期暴露中，温度升高会产生更大的镉积累。因此，earth在热应力（26°C）下暴露于125 mg Cd / kg的土壤中，其Cd积累量是19°C时的两倍。暴露于中等高浓度Cd（2.5–125 mg Cd / kg）并保持在26°C的worm显示出严重的体重减轻和高死亡率。从19暴露于最高Cd浓度的co中挤出的结肠细胞对中性红细胞的吸收能力在19°C下14 d后降低，在26°C下更为明显。在26°C下暴露于高于2.5 mg Cd / kg的浓度后以及在19°C下暴露于125 mg Cd / kg的浓度后，繁殖障碍（茧数减少）增加。在高温和亚致死浓度下，reproduction的繁殖能力极易受到有毒物质的影响。