Abstract The selection of cultivation conditions for enhancing the productivity and yields of key metabolites are essential for the economic viability of the microalgae-based biorefinery process. The effects of cultivation parameters on microalgal physiology are often species or strain specific. It is hence critical to establish baseline parameters for newly isolated strains and manipulate the culture conditions to optimise their metabolite yields. In this study, five microalgae strains isolated from the west of Ireland were cultivated under varying macronutrient concentrations (NaNO3 and NaH2PO4) and phytohormones (salicylic acid and methyl jasmonate) supplementation to establish their effects on growth rates, photosynthetic pigments and overall biochemical composition. Increasing the medium nitrate content 3-fold resulted in a significant increase in growth, pigments and lipids for 4 of the 5 strains. Methyl jasmonate supplementation caused an increase in carotenoids and lipids in all strains in a dose- and species-specify manner. Salicylic acid treatment induced an increase in protein content in Rhodella sp. APOT_15 and the two chlorophyta strains, K. aperta DMGFW_21 and B. submarina APSW_11. Overall, tailoring the cultivation conditions for each strain can lead to improved strain performance and the identification of key process parameters for the upscaled production of high-value metabolites.

中文翻译：

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补充营养和植物激素对新分离微藻生长、色素产量和生化成分的影响

摘要 选择培养条件以提高关键代谢物的生产力和产量对于微藻生物精炼工艺的经济可行性至关重要。培养参数对微藻生理的影响通常是物种或菌株特异性的。因此，为新分离的菌株建立基线参数并操纵培养条件以优化其代谢物产量至关重要。在这项研究中，从爱尔兰西部分离出的五种微藻菌株在不同的常量营养素浓度（NaNO3 和 NaH2PO4）和植物激素（水杨酸和茉莉酸甲酯）补充剂下培养，以确定它们对生长速率、光合色素和整体生化成分的影响。将培养基硝酸盐含量增加 3 倍导致 5 个菌株中的 4 个菌株的生长、色素和脂质显着增加。茉莉酸甲酯的补充导致所有菌株中类胡萝卜素和脂质以剂量和物种特定的方式增加。水杨酸处理诱导 Rhodella sp. 蛋白质含量增加。APOT_15 和两个叶绿素菌株 K. aperta DMGFW_21 和 B. submarina APSW_11。总体而言，为每个菌株定制培养条件可以提高菌株性能，并确定用于高价值代谢物大规模生产的关键工艺参数。水杨酸处理诱导 Rhodella sp. 蛋白质含量增加。APOT_15 和两个叶绿素菌株 K. aperta DMGFW_21 和 B. submarina APSW_11。总体而言，为每个菌株定制培养条件可以提高菌株性能，并确定用于高价值代谢物大规模生产的关键工艺参数。水杨酸处理诱导 Rhodella sp. 蛋白质含量增加。APOT_15 和两个叶绿素菌株 K. aperta DMGFW_21 和 B. submarina APSW_11。总体而言，为每个菌株定制培养条件可以提高菌株性能，并确定用于高价值代谢物大规模生产的关键工艺参数。