Abstract The optimum enzyme-assisted extraction conditions of cup plant (Silphium perfoliatum L.) polysaccharides (CPP) were obtained by response surface methodology (RSM), and were found to be an enzymatic hydrolysis time of 66 min, a liquid-solid ratio of 22 mL/g, pH 5.1, and an enzyme-complex dosage of 1.59 %, allowing a CPP yield of 13.69 %. DEAE-52 cellulose and Sepharose CL-6B columns were used to purify the CPP. A purified polysaccharide component, CPP-1.2, was obtained and further analyzed for its characteristics, antioxidant properties, and hypoglycemic activity. The monosaccharide composition of CPP-1.2 was mannose, galacturonic acid, glucose, galactose, and arabinose with a molar percentage ratio of 2.24:16.34:44.17:24.28:12.97. The 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2-Azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging abilities of CPP-1.2 (1.0 mg/mL) reached 95.20 % and 93.69 % that of ascorbic acid (1.0 mg/mL), respectively. Moreover, CPP-1.2 presented significantly higher inhibitory properties on the activities of α-amylase and α-glucosidase than acarbose at each concentration tested. These results suggest that CPP-1.2 possesses the potential to be used as an antioxidant and hypoglycemic agent.

中文翻译：

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一种杯形植物（Silphium perfoliatum L.）多糖的酶辅助提取及其抗氧化和降血糖活性

摘要 采用响应面法 (RSM) 确定了杯形植物 (Silphium perfoliatum L.) 多糖 (CPP) 的最佳酶辅助提取条件，酶解时间为 66 min，液固比为22 mL/g，pH 5.1，酶复合物用量为 1.59%，CPP 产率为 13.69%。DEAE-52 纤维素和 Sepharose CL-6B 柱用于纯化 CPP。获得了纯化的多糖成分 CPP-1.2，并进一步分析了其特性、抗氧化特性和降血糖活性。CPP-1.2的单糖组成为甘露糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖，摩尔百分比为2.24:16.34:44.17:24.28:12.97。1,1-二苯基-2-苦基肼 (DPPH) 和 2, CPP-1.2 (1.0 mg/mL) 的 2-Azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) 自由基清除能力达到抗坏血酸 (1.0 mg/mL) 的 95.20 % 和 93.69 % ， 分别。此外，在每个测试浓度下，CPP-1.2 对 α-淀粉酶和 α-葡萄糖苷酶的活性的抑制作用明显高于阿卡波糖。这些结果表明 CPP-1.2 具有用作抗氧化剂和降血糖剂的潜力。