Agricultural pest control is a popular research topic, and essential oils are widely studied because they represent a promising alternative to synthetic insecticides. However, despite the increase in studies on pests, little work has been done on pesticide contamination of the predators feeding on insecticide-affected prey. Therefore, the objective of this study was to evaluate the effects of Lethal Dose 50 (LD50) from the essential oils of Mentha spicata L. and Melaleuca alternifolia Cheel on the histology, including histochemistry (from protein and carbohydrate analysis) and immunohistochemistry (from the evaluation of cellular apoptosis), of the midgut of fifth instar nymphs of Podisus nigrispinus (stinkbug) (Dallas). The periods of analysis were 12, 24, and 48 h after ingestion of Alabama argillacea Hübner caterpillars treated with the respective oils. The oil from M. spicata did not cause histological alterations or apoptosis in the insect. However, there was a reduction in the level of carbohydrates within the 48-h period. After 24 h, the oil of M. alternifolia caused an elongation of digestive cells and, after 48 h, cell lysis with the release of material into the lumen, suggesting tissue necrosis. The immunohistochemical study revealed no apoptotic process. There was a reduction in the neutral carbohydrate levels in the 24- and 48-h periods and in the number of regenerative cells, when compared to the control, after the period of 48 h. These results demonstrate that M. spicata oil has potential for use in cotton fields because it does not affect the vital characteristics of P. nigrispinus. However, the essential oil of M. alternifolia is not suitable for use as a pesticide because it is extremely toxic to predators.

中文翻译：

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薄荷油和互花白千层香精油对黑Po（达拉斯）（半翅目：五翅目）中肠的影响。

农业害虫防治是一个受欢迎的研究主题，并且精油因其是合成杀虫剂的有前途的替代品而受到广泛研究。然而，尽管对害虫的研究有所增加，但对以杀虫剂影响的猎物为食的捕食者的杀虫剂污染却很少进行。因此，本研究的目的是评估薄荷（Mentha spicata L.）和互花白千层（Melaleuca alternifolia Cheel）精油中的致死剂量50（LD50）对组织学的影响，包括组织化学（来自蛋白质和碳水化合物分析）和免疫组织化学（来自（Palusus nigrispinus（臭鼬）（达拉斯）的五龄若虫的中肠）。分析周期为12、24，摄入分别用油处理的阿拉巴马州argillaceaHübner毛虫后48小时。辣根分枝杆菌的油没有引起昆虫的组织学改变或细胞凋亡。但是，在48小时内，碳水化合物的含量有所下降。24小时后，互生枝孢菌的油引起消化细胞伸长，48小时后，细胞裂解并向腔内释放物质，提示组织坏死。免疫组织化学研究显示无凋亡过程。与对照组相比，在48小时后的24小时和48小时内，中性碳水化合物水平降低了，再生细胞的数量也减少了。这些结果表明，辣根油在棉田中具有使用潜力，因为它不影响黑果松的生命特征。