Kohlrabi (Brassica oleracea L. var. gongylodes L.) was biofortified with selenium (Se), as selenite and selenate, and iodine (I), as iodide and iodate, and their combinations through foliar spraying, to study absorption of these elements by the plants, separately and in combination. The effects on selected physiological and morphological traits and optical characteristics were monitored. Treatments with Se positively affected total chlorophylls and carotenoids, and leaf stomata dimensions. Addition of I decreased total chlorophylls and increased anthocyanins. In reflectance spectra of the leaves, specific colour regions differed significantly due to the different treatments. Reflectance in the UV correlated positively with Se and I contents of the leaves, which indicated lower demand for production of phenolic compounds. Differences in reflectance in UV part of the spectra could be a consequence of changes in the cuticle. The Se and I levels increased markedly in leaves and tubers, without loss of mass or yield. Se had antagonistic effects on accumulation of I in leaves. The similar levels of Se and I in the leaves and tubers suggest that the transport of both elements in these plants occurs from the leaves to the tubers through the phloem. According to the Se and I contents in the kohlrabi tubers, biofortification with both elements simultaneously is feasible for human nutrition.

撇蓝（Brassica oleracea L. var。gongylodes用硒（Se）（亚硒酸盐和硒酸盐）和碘（I）（碘化物和碘酸盐）以及它们的组合通过叶面喷洒法进行了生物强化处理，以研究植物分别或组合吸收这些元素的情况。监测对选定的生理和形态特征以及光学特性的影响。硒处理对总叶绿素和类胡萝卜素以及叶片气孔尺寸有积极影响。I的添加减少了总叶绿素并增加了花色苷。在叶片的反射光谱中，由于不同的处理，特定的颜色区域显着不同。在紫外线下的反射率与叶片中的硒和硒含量呈正相关，这表明对酚类化合物生产的需求较低。光谱的紫外线部分的反射率差异可能是表皮变化的结果。叶片和块茎中的硒和硒水平显着增加，而没有质量或产量损失。硒对叶片中I的积累具有拮抗作用。叶片和块茎中Se和I的水平相似，表明这些植物中两种元素的转运都是通过韧皮部从叶片到块茎的。根据大头菜块茎中硒和硒的含量，同时进行两种元素的生物强化对人类营养是可行的。叶片和块茎中Se和I的水平相似，表明这些植物中两种元素的转运都是通过韧皮部从叶片到块茎的。根据大头菜块茎中硒和硒的含量，同时进行两种元素的生物强化对人类营养是可行的。叶片和块茎中Se和I的水平相似，表明这些植物中两种元素的转运都是通过韧皮部从叶片到块茎的。根据大头菜块茎中硒和硒的含量，同时进行两种元素的生物强化对人类营养是可行的。