Bacteriophage endolysin is one of the potential alternatives of conventional antibiotics, but the intrinsic limitations of the bacterial expression system may undermine the comprehensive application of this therapeutic protein. To circumvent such limitations, we adopted a yeast surface display system as a novel expression platform for endolysin. Endolysin LysSA11 from staphylococcal phage SA11 was expressed and surface-displayed in Saccharomyces cerevisiae to exhibit sufficient antimicrobial activity against Staphylococcus aureus. Without any protein isolation or purification procedures, we showed that direct treatment of LysSA11-displaying yeast cells could accomplish a 5-log reduction of viable Staphylococcus aureus within 3 h. Furthermore, the surface-displayed LysSA11 exhibited superior stability over the soluble form of purified LysSA11 during 14 days of storage in a refrigerated environment. We suggest that the yeast surface display system is an efficient, stable, and straightforward platform for the production and antibacterial applications of endolysin.

中文翻译：

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酵母表面展示系统，可促进噬菌体内溶素的生产和应用。

噬菌体溶血素是常规抗生素的潜在替代品之一，但细菌表达系统的固有局限性可能会破坏这种治疗性蛋白质的全面应用。为了避免这种局限性，我们采用了酵母表面展示系统作为内溶素的新型表达平台。来自酿酒葡萄球菌噬菌体SA11的内溶素LysSA11在酿酒酵母中表达和表面展示，以表现出对金黄色葡萄球菌足够的抗菌活性。没有任何蛋白质分离或纯化程序，我们显示了直接处理展示LysSA11的酵母细胞可以在3 h内使活的金黄色葡萄球菌减少5个对数。此外，表面展示的LysSA11在冷藏环境中存放14天时，表现出优于纯化的LysSA11可溶性形式的稳定性。我们建议，酵母表面展示系统是一种高效，稳定和直接的平台，用于生产和溶菌素。