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Biomineral Precursor Formation Is Initiated by Transporting Calcium and Phosphorus Clusters from the Endoplasmic Reticulum to Mitochondria.
Advanced Science ( IF 14.3 ) Pub Date : 2020-03-02 , DOI: 10.1002/advs.201902536 Cuizhu Tang 1, 2 , Yan Wei 1, 2 , Lin Gu 3 , Qinghua Zhang 3 , Mei Li 4 , Guohua Yuan 1, 2 , Yuan He 5 , Li Huang 5 , Yan Liu 6 , Yufeng Zhang 1, 2
Advanced Science ( IF 14.3 ) Pub Date : 2020-03-02 , DOI: 10.1002/advs.201902536 Cuizhu Tang 1, 2 , Yan Wei 1, 2 , Lin Gu 3 , Qinghua Zhang 3 , Mei Li 4 , Guohua Yuan 1, 2 , Yuan He 5 , Li Huang 5 , Yan Liu 6 , Yufeng Zhang 1, 2
Affiliation
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Mineral granules in the mitochondria of bone-forming cells are thought to be the origin of biomineral precursors, which are transported to extracellular matrices to initiate cell-mediated biomineralization. However, no evidence has revealed how mitochondrial granules form. This study indicates that mitochondrial granules are initiated by transporting calcium and phosphorus clusters from the endoplasmic reticulum (ER) to mitochondria based on detailed observations of the continuous process of mouse parietal bone development as well as in vitro biomineralization in bone-forming cells. Nanosized biomineral precursors (≈30 nm in diameter), which originate from mitochondrial granules, initiate intrafibrillar mineralization of collagen as early as embryonic day 14.5. Both in vivo and in vitro studies further reveal that formation of mitochondrial granules is induced by the ER. Elevated levels of intracellular calcium or phosphate ions, which can be induced by treatment with ionomycin and black phosphorus, respectively, accelerate formation of the calcium and phosphorus clusters on ER membranes and ultimately promote biomineralization. These findings provide a novel insight into biomineralization and bone formation.
中文翻译：
生物矿物质前体的形成是通过将钙和磷簇从内质网运输到线粒体来启动的。
成骨细胞线粒体中的矿物质颗粒被认为是生物矿物质前体的起源，生物矿物质前体被转运到细胞外基质以启动细胞介导的生物矿化。然而，没有证据表明线粒体颗粒是如何形成的。本研究基于对小鼠顶骨发育的连续过程以及成骨细胞的体外生物矿化的详细观察，表明线粒体颗粒是通过将钙和磷簇从内质网（ER）转运到线粒体而启动的。纳米级生物矿物质前体（直径约 30 nm）源自线粒体颗粒，早在胚胎第 14.5 天就启动胶原蛋白的纤维内矿化。体内和体外研究进一步表明线粒体颗粒的形成是由内质网诱导的。分别用离子霉素和黑磷处理可诱导细胞内钙或磷酸根离子水平升高，加速内质网膜上钙和磷簇的形成，最终促进生物矿化。这些发现为生物矿化和骨形成提供了新的见解。
更新日期：2020-04-21
中文翻译：
生物矿物质前体的形成是通过将钙和磷簇从内质网运输到线粒体来启动的。
成骨细胞线粒体中的矿物质颗粒被认为是生物矿物质前体的起源，生物矿物质前体被转运到细胞外基质以启动细胞介导的生物矿化。然而，没有证据表明线粒体颗粒是如何形成的。本研究基于对小鼠顶骨发育的连续过程以及成骨细胞的体外生物矿化的详细观察，表明线粒体颗粒是通过将钙和磷簇从内质网（ER）转运到线粒体而启动的。纳米级生物矿物质前体（直径约 30 nm）源自线粒体颗粒，早在胚胎第 14.5 天就启动胶原蛋白的纤维内矿化。体内和体外研究进一步表明线粒体颗粒的形成是由内质网诱导的。分别用离子霉素和黑磷处理可诱导细胞内钙或磷酸根离子水平升高，加速内质网膜上钙和磷簇的形成，最终促进生物矿化。这些发现为生物矿化和骨形成提供了新的见解。
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