An alternative mechanism of early nodal clustering and myelination onset in GABAergic neurons of the central nervous system.,Glia

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An alternative mechanism of early nodal clustering and myelination onset in GABAergic neurons of the central nervous system.
Glia ( IF 5.4 ) Pub Date : 2020-03-02 , DOI: 10.1002/glia.23812
Melina Thetiot ₁ , Sean A Freeman _{1,

2} , Thomas Roux _{1,

2} , Anne-Laure Dubessy _{1,

2} , Marie-Stéphane Aigrot ₁ , Quentin Rappeneau ₃ , François-Xavier Lejeune ₁ , Julien Tailleur ₄ , Nathalie Sol-Foulon ₁ , Catherine Lubetzki _{1,

2} , Anne Desmazieres ₁

Affiliation

In vertebrates, fast saltatory conduction along myelinated axons relies on the node of Ranvier. How nodes assemble on CNS neurons is not yet fully understood. We previously described that node‐like clusters can form prior to myelin deposition in hippocampal GABAergic neurons and are associated with increased conduction velocity. Here, we used a live imaging approach to characterize the intrinsic mechanisms underlying the assembly of these clusters prior to myelination. We first demonstrated that their components can partially preassemble prior to membrane targeting and determined the molecular motors involved in their trafficking. We then demonstrated the key role of the protein β2Na_v for node‐like clustering initiation. We further assessed the fate of these clusters when myelination proceeds. Our results shed light on the intrinsic mechanisms involved in node‐like clustering prior to myelination and unravel a potential role of these clusters in node of Ranvier formation and in guiding myelination onset.

中文翻译：

中枢神经系统 GABA 能神经元早期节点聚集和髓鞘形成的另一种机制。

在脊椎动物中，沿着有髓轴突的快速跳跃传导依赖于 Ranvier 节点。节点如何在 CNS 神经元上组装尚不完全清楚。我们之前描述了节点状簇可以在海马 GABA 能神经元中的髓鞘沉积之前形成，并且与传导速度增加有关。在这里，我们使用实时成像方法来表征这些簇在髓鞘形成之前组装的内在机制。我们首先证明了它们的组件可以在膜靶向之前部分预组装，并确定了参与其贩运的分子马达。然后我们证明了蛋白质 β2Na _v的关键作用用于类似节点的聚类启动。我们进一步评估了髓鞘形成时这些簇的命运。我们的研究结果阐明了髓鞘形成之前节点状聚类所涉及的内在机制，并阐明了这些簇在 Ranvier 形成节点和指导髓鞘形成中的潜在作用。

更新日期：2020-03-02

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