Efficient Nicotinamide Adenine Dinucleotide Phosphate [NADP(H)] Recycling in Closed‐Loop Continuous Flow Biocatalysis,Advanced Synthesis & Catalysis

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Efficient Nicotinamide Adenine Dinucleotide Phosphate [NADP(H)] Recycling in Closed‐Loop Continuous Flow Biocatalysis
Advanced Synthesis & Catalysis ( IF 4.4 ) Pub Date : 2020-03-01 , DOI: 10.1002/adsc.202000058
Benedikt Baumer ₁ , Thomas Classen ₂ , Martina Pohl ₂ , Jörg Pietruszka _{1,

2}

Affiliation

Biocatalytic redox reactions regularly depend on expensive cofactors that require recycling. For continuous conversions in flow chemistry, this is often an obstacle since the cofactor is washed away. Here, we present a quasi‐stationary recycling system for nicotinamide adenine dinucleotide phosphate utilizing an immobilized alcohol dehydrogenase. Four model substrates were reduced with high enantioselectivity as a proof of concept. The two‐phase system enables continuous production as well as quick substrate changes. This setup may serve as a general cofactor regeneration module for continuous biocatalytic devices employing (co‐)substrates being miscible in organic solvent. The system resulted in space‐time yields up to 117 g L⁻¹ h⁻¹ and total turnover numbers for nicotinamide adenine dinucleotide phosphate higher than 12,000 mol/mol are possible.

中文翻译：

闭环连续流生物催化中高效烟酰胺腺嘌呤二核苷酸磷酸酯[NADP（H）]的回收利用

生物催化氧化还原反应通常取决于需要回收的昂贵辅因子。对于流动化学的连续转化，这通常是一个障碍，因为辅因子被冲走了。在这里，我们介绍一种利用固定化醇脱氢酶的烟酰胺腺嘌呤二核苷酸磷酸准静态回收系统。以高对映选择性还原了四种模型底物作为概念证明。两阶段系统可实现连续生产以及快速更换基材。该设置可以用作使用（共）底物可混溶在有机溶剂中的连续生物催化设备的通用辅助因子再生模块。该系统的时空产量高达117 g L ^-1 h ^-1 烟酰胺腺嘌呤二核苷酸磷酸的总营业额可能高于12,000 mol / mol。

更新日期：2020-03-01

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