Abstract An environmentally friendly field-portable approach based on small-scale ultrasound-assisted extraction and micro-volume colorimetry was proposed for the determination of iron using natural reagent extracts from pomegranate peels. Ultrasound technology was utilized for the extraction of pomegranate peels in acetate buffer pH 5.5. Sonication parameters influencing the extraction process of natural reagents from pomegranate peel were investigated, including the type of solvent, mass/volume ratio of solvent, sonication time, temperature, and power. The efficiency of the ultrasound-assisted procedure for the extraction of iron from pharmaceutical formulations was evaluated by comparing to their label value and the reference method for pharmaceutical formulations. A linear calibration graph ranging from 1.0 to 10.0 mg L−1 Fe(III) was obtained from micro-volume colorimetric determination (at 530 nm) with limits of detection and quantification of 0.31 and 0.78 mg L−1, respectively. The reproducibility for 3.0 mg L−1 Fe(III) was 3.8% as the relative standard deviation. This developed system was successfully applied to determine the iron content in pharmaceutical formulations and the results agreed well with those obtained by a reference method. Good recoveries between 97 and 105% were obtained.

中文翻译：

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石榴皮中酚类物质的小规模超声辅助提取及其作为天然试剂在铁比色测定中的应用

摘要 提出了一种基于小规模超声辅助提取和微体积比色法的环保现场便携式方法，用于使用石榴皮天然试剂提取物测定铁。利用超声波技术在 pH 5.5 的醋酸盐缓冲液中提取石榴皮。研究了影响石榴皮天然试剂提取过程的超声处理参数，包括溶剂类型、溶剂的质量/体积比、超声处理时间、温度和功率。通过与药物制剂的标签值和参考方法进行比较，评估了从药物制剂中提取铁的超声辅助程序的效率。范围从 1.0 到 10 的线性校准图。0 mg L-1 Fe(III) 是通过微量比色测定（530 nm）获得的，检测限和定量限分别为 0.31 和 0.78 mg L-1。作为相对标准偏差，3.0 mg L-1 Fe(III) 的重现性为 3.8%。该开发的系统已成功应用于确定药物制剂中的铁含量，其结果与参考方法获得的结果非常吻合。获得了 97% 到 105% 的良好回收率。该开发的系统已成功应用于确定药物制剂中的铁含量，其结果与参考方法获得的结果非常吻合。获得了 97% 到 105% 的良好回收率。该开发的系统已成功应用于确定药物制剂中的铁含量，其结果与参考方法获得的结果非常吻合。获得了 97% 到 105% 的良好回收率。