Horseradish peroxidase (HRP) is a classic cis-diol-containing glycoprotein and can be extracted from horseradish plants; it has low loading capacity and enzyme stability in the separation process. Functionalized magnetic nanoparticles have been proved to be effective for the separation and purification of glycoproteins. In this work, dendrimeric phenylboronic acid-functionalized magnetic graphene oxide nanoparticles (d-PBA-GO@Fe₃O₄@PEI) were synthesized via dendrimer-assisted multivalent synergistic binding. The boronic acid in the dendrimeric magnetic nanoparticles provided docking sites for cis-diol-containing biomolecules. d-PBA-GO@Fe₃O₄@PEI exhibited excellent performance in the separation of HRP with a binding capacity as high as 557.75 mg g⁻¹. The magnetic nanoparticles also exhibited excellent recyclability. It was found that the nanoparticles had satisfactory performance for the separation and purification of HRP from the crude extract of horseradish; moreover, the HRP structure remained unchanged before and after the elution process. The outstanding adsorption performance (high adsorption capacity and specific recognition) makes the nanoparticles promising for the separation and purification of glycoproteins in the future industrial production.

中文翻译：

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具有良好吸附性能的树枝状苯基硼酸官能化磁性氧化石墨烯纳米颗粒的制备，用于分离和纯化辣根过氧化物酶

辣根过氧化物酶（HRP）是一种经典的含顺式二醇的糖蛋白，可以从辣根植物中提取；它在分离过程中具有较低的负载能力和酶稳定性。已经证实功能化的磁性纳米颗粒对于糖蛋白的分离和纯化是有效的。在这项工作中，通过树枝状聚合物辅助的多价协同结合合成了树枝状的苯基硼酸官能化的氧化石墨烯纳米粒子（d-PBA-GO @ Fe ₃ O ₄ @PEI）。树枝状磁性纳米颗粒中的硼酸为含顺式-二醇的生物分子提供了对接位点。d-PBA-GO @ Fe ₃ O ₄@PEI在HRP分离中表现出优异的性能，结合能力高达557.75 mg g ^-1。磁性纳米颗粒还表现出优异的可回收性。发现该纳米颗粒具有从辣根粗提物中分离和纯化HRP的令人满意的性能。此外，在洗脱过程之前和之后，HRP结构保持不变。出色的吸附性能（高吸附容量和特异性识别）使纳米颗粒有望在未来的工业生产中用于糖蛋白的分离和纯化。