The centrosome linker serves to hold the duplicated centrosomes together until they separate in late G2/early mitosis. Precisely how the linker is assembled remains an open question. In this study, we identify Cep44 as a novel component of the linker in human cells. Cep44 localizes to the proximal end of centrioles, including mother and daughter centrioles, and its ablation leads to loss of centrosome cohesion. Cep44 does not impinge on the stability of C-Nap1 (also known as CEP250), LRRC45 or Cep215 (also known as CDK5RAP2), and vice versa, and these proteins are independently recruited to the centrosome. Rather, Cep44 associates with rootletin and regulates its stability and localization to the centrosome. Our findings reveal a role of the previously uncharacterized protein Cep44 for centrosome cohesion and linker assembly.

中文翻译：

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Cep44 通过稳定 rootletin 发挥中心体凝聚力的作用。


中心体连接体用于将复制的中心体保持在一起，直到它们在 G2 晚期/有丝分裂早期分离。链接器到底是如何组装的仍然是一个悬而未决的问题。在这项研究中，我们将 Cep44 确定为人类细胞连接子的新成分。 Cep44定位于中心粒的近端，包括母中心粒和子中心粒，其消融导致中心体内聚力丧失。 Cep44 不会影响 C-Nap1（也称为 CEP250）、LRRC45 或 Cep215（也称为 CDK5RAP2）的稳定性，反之亦然，并且这些蛋白质独立地募集到中心体。相反，Cep44 与 rootletin 结合并调节其稳定性和中心体定位。我们的研究结果揭示了先前未表征的蛋白质 Cep44 在中心体凝聚和接头组装中的作用。