Round scad (Decapterus maruadsi) is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, there is no comprehensive information covering its lipidomic profile. In this study, lipid profiles including fatty acid composition, distribution and detailed structure information were explored using gas chromatography-flame ionization detector (GC-FID) and ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap-MS). The results showed that triacylglycerol (TG) was the dominant class, and the EPA and DHA were concentrated as glycerophospholipid (GP). The saturated fatty acids and monounsaturated fatty acids were mainly esterified in positions 1 and 3 of TG and sn-1-position of GP, while the polyunsaturated fatty acids (PUFA) were mainly esterified in sn-2-position of TG and GP. A total of 1282 species from six classes (21 subclasses) including glycerolipids (GL), sphingolipids (SP), GP, fatty acyls (FA), saccharolipids (SL) and prenol lipids (PR) were identified. Several molecular species were characterized with PUFA, especially EPA and DHA in GP. Considering the superior fatty acids composition and distribution of round scad, it is deserved for further exploitation of its marine lipid source on account of the healthy and nutritional functions.

圆鳞（Decapterus maruadsi）富含二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）。但是，没有涵盖其脂质组学概况的全面信息。在这项研究中，使用气相色谱-火焰电离检测器（GC-FID）和超高效液相色谱结合混合四极-轨道质谱仪（UPLC-Q）探索了包括脂肪酸组成，分布和详细结构信息在内的脂质谱-Exactive Orbitrap-MS）。结果表明，三酰基甘油（TG）是主要的类别，EPA和DHA被浓缩为甘油磷脂（GP）。饱和脂肪酸和单不饱和脂肪酸主要在TG的1和3位以及GP的sn-1位被酯化，而多不饱和脂肪酸（PUFA）主要在TG和GP的sn-2位置酯化。总共鉴定了来自六个类别（21个亚类）的1282种，包括甘油脂（GL），鞘脂（SP），GP，脂肪酰基（FA），糖脂（SL）和prenol脂质（PR）。PUFA表征了几种分子，尤其是GP中的EPA和DHA。考虑到圆形脂肪酸的优越的脂肪酸组成和分布，由于其健康和营养功能，应进一步开发其海洋脂质来源。