Abstract This research aimed to demonstrate the significance of bioaugmentation in the mycoremediation of metals and metalloid (Cr, Cu, As, Fe, Mn) polluted landfill soil using consortia of autochthonous filamentous fungi. The fungal consortia used were Ascomycota, all isolated fungi, and Basidiomycota. Bioremediation was monitored for pH, redox potential, electrical conductivity, residual heavy metal/metalloid content, fungal population and enzyme activity at day 0 (initial day), day 20, day 60 and day 100. Results have shown a decreasing trend for all the monitored physicochemical parameters. Fungal organisms have a maximum tolerance index of 1.0 on Fe, Cu and Cr supplemented Agar medium. Highest colony count of 1.17 × 1010 CFU/g soil was recorded in Basidiomycota treated soil. On the other hand, a consortium of all isolated fungi proved efficient in the removal of As(77 %), Mn(71 %), Cr(60 %), and Cu(52 %). Meanwhile, Fe removal of 56 % was prioritized by the Ascomycota consortium. Acid phosphatase had the weakest activity (0.03 – 0.72 μmol PNPg−1 dry soil h−1) for all the treatments. FTIR results have shown the appearance of absorption peaks at 1485 - 1445 cm−1 only in soil amended with fungal consortia. Fungi bioaugmented soil had the maximum metal bio removal efficiency than the untreated control soil (P

中文翻译：

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使用丝状真菌联盟的重金属和/类金属垃圾填埋场污染土壤的生物强化辅助真菌介导

摘要 本研究旨在证明生物强化在利用本地丝状真菌财团对金属和准金属（Cr、Cu、As、Fe、Mn）污染的垃圾填埋场土壤进行真菌修复中的意义。使用的真菌聚生体是子囊菌门、所有分离的真菌和担子菌门。在第 0 天（初始天）、第 20 天、第 60 天和第 100 天监测生物修复的 pH、氧化还原电位、电导率、残留重金属/类金属含量、真菌种群和酶活性。监测理化参数。真菌生物体对 Fe、Cu 和 Cr 补充琼脂培养基的最大耐受指数为 1.0。在担子菌处理的土壤中记录的最高菌落数为 1.17 × 1010 CFU/g 土壤。另一方面，证明所有分离真菌的联合体在去除 As(77%)、Mn(71%)、Cr(60%) 和 Cu(52%) 方面是有效的。同时，子囊菌联盟优先考虑去除 56% 的铁。对于所有处理，酸性磷酸酶的活性最弱（0.03 – 0.72 μmol PNPg-1 干燥土壤 h-1）。FTIR 结果显示仅在用真菌聚生体修正的土壤中出现 1485 - 1445 cm-1 的吸收峰。真菌生物增强土壤比未处理的对照土壤具有最大的金属生物去除效率（P FTIR 结果显示仅在用真菌聚生体修正的土壤中出现 1485 - 1445 cm-1 的吸收峰。真菌生物增强土壤比未处理的对照土壤具有最大的金属生物去除效率（P FTIR 结果显示仅在用真菌聚生体修正的土壤中出现 1485 - 1445 cm-1 的吸收峰。真菌生物增强土壤比未处理的对照土壤具有最大的金属生物去除效率（P