A triclosan-resistance protein from the soil metagenome is a novel enoyl-acyl carrier protein reductase: Structure-guided functional analysis.,The FEBS Journal

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A triclosan-resistance protein from the soil metagenome is a novel enoyl-acyl carrier protein reductase: Structure-guided functional analysis.
The FEBS Journal ( IF 5.5 ) Pub Date : 2020-02-28 , DOI: 10.1111/febs.15267
Sang-Hoon Kim ₁ , Raees Khan _{2,

3} , Kihyuck Choi ₂ , Seon-Woo Lee ₂ , Sangkee Rhee _{1,

4}

Affiliation

The synthetic biocide triclosan targets enoyl‐acyl carrier protein reductase(s) (ENR) in bacterial type II fatty acid biosynthesis. Screening and sequence analyses of the triclosan resistome from the soil metagenome identified a variety of triclosan‐resistance ENRs. Interestingly, the mode of triclosan resistance by one hypothetical protein was elusive, mainly due to a lack of sequence similarity with other proteins that mediate triclosan resistance. Here, we carried out a structure‐based function prediction of the hypothetical protein, herein referred to as FabMG, and in vivo and in vitro functional analyses. The crystal structure of FabMG showed limited structural homology with FabG and FabI, which are also involved in type II fatty acid synthesis. In vivo complementation and in vitro activity assays indicated that FabMG is functionally a FabI‐type ENR that employs NADH as a coenzyme. Variations in the sequence and structure of FabMG are likely responsible for inefficient binding of triclosan, resulting in triclosan resistance. These data unravel a previously uncharacterized FabMG, which is prevalent in various microbes in triclosan‐contaminated environments and provide mechanistic insight into triclosan resistance.

中文翻译：

来自土壤基因组的三氯生抗性蛋白是一种新型烯酰酰基载体蛋白还原酶：结构指导的功能分析。

合成的杀生物剂三氯生靶向细菌II型脂肪酸生物合成中的烯酰基-酰基载体蛋白还原酶（ENR）。从土壤基因组中对三氯生抗药性基因组进行筛选和序列分析，发现了多种三氯生抗性ENR。有趣的是，一种假设的蛋白对三氯生的抗性模式难以捉摸，这主要是由于与介导三氯生抗性的其他蛋白缺乏序列相似性。在这里，我们对假设的蛋白质（在此称为FabMG）进行了基于结构的功能预测，并进行了体内和体外功能分析。FabMG的晶体结构显示与FabG和FabI的结构同源性有限，而FabG和FabI也参与II型脂肪酸合成。体内互补和体外活性分析表明，FabMG在功能上是采用NADH作为辅酶的FabI型ENR。FabMG的序列和结构的变化可能是导致三氯生结合效率低下的原因，导致了三氯生抗药性。这些数据揭示了以前未表征的FabMG，FabMG在三氯生污染的环境中广泛存在于各种微生物中，并提供了对三氯生抗性的机理性见解。

更新日期：2020-02-28

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