ABSTRACT This study aimed to develop and validate a mathematical model for predicting ozone propagation and distribution in a bench-scale storage silo of soybean and link the model results to its application as an antifungal. The reduction of potentially toxigenic fungi found in soybeans was also evaluated after the ozone treatment. Experimental validation consisted of the ozone application and concentration (ozone) monitoring at the experimental silo outlet. The soybean grains were analyzed prior to and after ozone treatment from different portions of the silo to evaluate the antifungal activity. After 30 minutes of ozone exposure, there was an 88% reduction in the amount of fungi present at the lower section, reaching total elimination with longer exposure times. Fusarium was the main genus found, and it was significantly reduced even with the shortest exposure (30 min). The results of the ozone propagation in soybeans will be useful to better understand its behavior within silos, in order for the elimination of biological contaminations to occur. The model showed ozone spread through the silo, and this treatment showed to be an effective alternative to control against potentially toxigenic fungi found in soybeans.

中文翻译：

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大豆筒仓臭氧除霉建模与试验

摘要 本研究旨在开发和验证一个数学模型，用于预测大豆实验室规模储存筒仓中臭氧的传播和分布，并将模型结果与其作为抗真菌剂的应用联系起来。臭氧处理后还评估了大豆中潜在产毒真菌的减少情况。实验验证包括实验筒仓出口处的臭氧应用和浓度（臭氧）监测。在臭氧处理之前和之后对来自筒仓不同部分的大豆粒进行分析，以评估抗真菌活性。在臭氧暴露 30 分钟后，下部的真菌数量减少了 88%，随着暴露时间的延长，达到完全消除。镰刀菌是发现的主要属，即使在最短的曝光时间（30 分钟）下，它也显着降低。臭氧在大豆中传播的结果将有助于更好地了解其在筒仓内的行为，从而消除生物污染。该模型显示臭氧通过筒仓扩散，这种处理表明是控制大豆中发现的潜在有毒真菌的有效替代方法。