This study compared metabolite shifts induced by training for, participation in, and recovery from a marathon race competition among athletes divided into three groups based on fitness (relative maximum oxygen uptake (VO2max)) and performance levels (net running time). Plasma samples from 76 male runners participating in the Munich Marathon were analyzed for metabolite shifts using a targeted metabolomics panel. For the entire cohort of runners, pronounced increases were measured immediately after the race for plasma concentrations of acylcarnitines (AC), the ratio (palmitoylcarnitine + stearoylcarnitine)/free carnitine that is used as a proxy for the activity of the mitochondrial enzyme carnitine palmitoyltransferase, and arginine-related metabolites, with decreases in most amino acids (AA) and phospholipids. Plasma levels of AA and phospholipids were strongly increased 24 and 72 h post-race. Post-race plasma concentrations of AC and arginine-related metabolites were higher in the low compared to top performers, indicating an accumulation of fatty acids and a reliance on protein catabolism to provide energy after the marathon event. This study showed that marathon race competition is associated with an extensive and prolonged perturbation in plasma metabolite concentrations with a strong AC signature that is greater in the slower, less aerobically fit runners. Furthermore, changes in the arginine-related metabolites were observed.

中文翻译：

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马拉松比赛引起的代谢变化因运动员的体能和表现水平而异：Enzy-MagIC研究的一个子研究。

这项研究比较了运动员的马拉松比赛训练，参加和从马拉松比赛中恢复所引起的代谢变化，根据体能（最大摄氧量（VO2max））和体能水平（净跑步时间）分为三组。使用目标代谢组学小组分析了参加慕尼黑马拉松赛的76名男运动员的血浆样品的代谢物变化。对于整个跑步者来说，比赛后血浆酰基肉碱（AC）浓度即比例（棕榈酰肉碱+硬脂酰肉碱）/游离肉碱（代表线粒体酶肉碱棕榈酰转移酶的活性）的比率随即显着增加，和精氨酸相关的代谢产物，大多数氨基酸（AA）和磷脂减少。血浆AA和磷脂水平在赛后24和72 h显着增加。赛后血浆中AC和精氨酸相关代谢物的浓度与性能最佳者相比较低，这表明马拉松事件后脂肪酸的积累和蛋白质分解代谢的依赖以提供能量。这项研究表明，马拉松比赛与血浆代谢物浓度的广泛且长时间的扰动有关，具有较强的AC信号，而慢速，需氧能力较弱的运动员则表现出更大的AC信号。此外，观察到精氨酸相关代谢产物的变化。表明在马拉松比赛后脂肪酸积累并依赖蛋白质分解代谢提供能量。这项研究表明，马拉松比赛与血浆代谢物浓度的广泛和长期扰动有关，具有很强的AC信号，在速度较慢，需氧能力较弱的运动员中更大。此外，观察到精氨酸相关代谢产物的变化。表明在马拉松比赛后脂肪酸积累并依赖蛋白质分解代谢提供能量。这项研究表明，马拉松比赛与血浆代谢物浓度的广泛且长时间的扰动有关，具有较强的AC信号，而慢速，需氧能力较弱的运动员则表现出更大的AC信号。此外，观察到精氨酸相关代谢产物的变化。