The industrial ethanol fermentation imposes several stresses to microorganisms. However, some bacterial species are well adapted and manage to endure these harmful conditions. Lactobacillus vini is one of the most found bacteria in these environments, indicating the existence of efficient tolerance mechanisms. In view of this premise, the present study aimed to describe the tolerance of L. vini to several stressing agents encounter in industrial environments and the genetic components of the stress response. In general, L. vini showed significant tolerance to stressors commonly found in fuel-ethanol fermentations, and only doses higher than normally reached in processes restrained its growth. The lag phase and the growth rate were the most responsive kinetic parameter affected. Gene expression analysis revealed that uspII gene positively responded to all conditions tested, a typical profile of a general stress response gene. In addition, the results also revealed aspects of regulatory modules of co-expressed genes responding to different stresses, and also the similarities of response mechanism with basis in common cellular damages. Altogether, these data contribute to uncover the factors that could favour L. vini in the industrial fermentation which could be shared with other well adapted species and reports the first stress response genes in this bacterium.

工业乙醇发酵对微生物施加了多种压力。但是，某些细菌种类适应性强，可以忍受这些有害条件。葡萄乳杆菌是在这些环境中发现最多的细菌之一，表明存在有效的耐受机制。鉴于此前提下，旨在的公差描述本研究L. VINI几个强调剂在工业环境中和应激反应的遗传组分遇到。一般来说，L。vini对燃料乙醇发酵中常见的应激源显示出显着的耐受性，只有高于正常过程中达到的剂量才能抑制其生长。滞后阶段和生长速率是受影响最敏感的动力学参数。基因表达分析表明，uspII基因对所有测试条件均呈阳性反应，这是一般应激反应基因的典型特征。此外，结果还揭示了共表达基因对不同压力的反应的调控模块的各个方面，以及常见细胞损伤中反应机制与基础的相似性。总而言之，这些数据有助于发现可能有利于维氏乳杆菌的因素。 在工业发酵中可以与其他适应性强的物种共享，并报告了该细菌的第一个应激反应基因。