Irreversible electroporation holds great potential for cell-specific lysis due to the size-dependent susceptibility of cells to externally imposed electric fields. Previous attempts at selective cell lysis lead to significant overlap between affected populations and struggle with inconsistent biological outcome. We propose that charge transfer at the electrode-liquid interface is responsible by inducing multifactorial effects originating from both the electric field and electrochemical reactions. A promising remedy is the coating of electrodes with a high-k dielectric layer. The resulting capacitive coupling restores the selective potential of electric field mediated lysis in a microfluidic setup. Initial experiments show the consistent depletion of erythrocytes from whole blood while leaving leukocytes intact. The same is true for the reproducible and selective depletion of Jurkat and MCF-7 cells in a mixture with leukocytes. Unexpectedly, the observed order of lysis cannot be correlated with cell size. This implies that the cellular response to capacitive coupling features a selective characteristic that is different from conventional lysis configurations.

中文翻译：

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电容耦合提高了电场破坏细胞特异性肿瘤的准确性。

由于细胞对外部施加的电场的大小依赖性，不可逆电穿孔在细胞特异性裂解方面具有巨大潜力。先前对选择性细胞裂解的尝试导致了受影响人群之间的重大重叠，并与生物学结果不一致产生了争执。我们提出，在电极-液体界面处的电荷转移是通过引起源自电场和电化学反应的多因素效应来负责的。一种有前途的补救方法是在电极上涂一层高k介电层。所得的电容性耦合在微流体装置中恢复了电场介导的裂解的选择性电势。最初的实验表明，全血中的红细胞持续耗竭，而白细胞保持完整。对于与白细胞混合的Jurkat和MCF-7细胞，其可复制和选择性消耗也是如此。出乎意料的是，观察到的裂解顺序无法与细胞大小相关。这意味着细胞对电容耦合的响应具有不同于常规裂解配置的选择性特征。