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Heterogeneous expression of the ammonium transporter AgAmt in chemosensory appendages of the malaria vector, Anopheles gambiae.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-02-29 , DOI: 10.1016/j.ibmb.2020.103360
Zi Ye 1 , Feng Liu 1 , Huahua Sun 1 , Mackenzie Barker 2 , R Jason Pitts 3 , Laurence J Zwiebel 1
Affiliation  

Ammonia is one of the principal kairomones originating from human and other animal emanations and in that context, plays an essential role in the host-seeking behaviors of the malaria vector mosquito Anopheles gambiae. Nevertheless, despite its importance in directing host-seeking, the mechanisms underlying ammonia detection in the mosquito olfactory system remains largely unknown. In addition to ongoing efforts to identify and characterize the molecular receptors that underlie ammonia sensitivity, previous studies have revealed a prominent role for ammonium transporters (Amt) in modulating antennal and behavioral responses in Drosophila melanogaster and An. gambiae. In the former, localization of DmAmt in antennal sensilla to auxiliary cells surrounding the ammonia sensory neurons led to the hypothesis that its role was to clear excess ammonium ions in the sensillar lymph. In the latter, RT-PCR and heterologous expression have been used to examine the expression and functional characteristics of the An. gambiae ammonium transporter, AgAmt. We now employ advanced transgenic tools to comprehensively examine AgAmt spatial localization across the peripheral chemosensory appendages in larvae and adult female An. gambiae. In the larval antennae, AgAmt appears localized in both neuronal and auxiliary cells. In contrast to D. melanogaster, in the adult antennae, AgAmt-derived signals are observed in both non-neuronal auxiliary cells and in sensory neurons in ammonia-responsive basiconic and coeloconic sensilla. In the maxillary palps, labella, and tarsi, AgAmt appears restricted to sensory neurons. We have also characterized the responses to ammonia of adult antennal coeloconic sensilla and maxillary palp capitate pegs revealing a correlation between sensillar AgAmt expression and ammonia sensitivity. Taken together, these data suggest that AgAmt may play heterogeneous roles in the adult and larval chemosensory apparatus and potentially broad utility as a supra-receptor target in mosquito control.

中文翻译:

铵转运蛋白AgAmt在疟疾媒介冈比亚按蚊的化学感觉附属物中的异质表达。

氨是源自人类和其他动物排放物的主要海洛酮,在这种情况下,它在疟疾媒介冈比亚按蚊中寻找宿主的行为中起着至关重要的作用。尽管如此,尽管它在指导寻找宿主方面很重要,但在蚊香系统中检测氨的机制仍很未知。除了不断努力鉴定和表征氨敏感性基础的分子受体外,先前的研究还揭示了铵转运蛋白(Amt)在调节果蝇和黑腹果蝇的触角和行为反应中的重要作用。冈比亚 在前者中 DmAmt在触角感觉器中定位为氨感觉神经元周围的辅助细胞导致了这样一个假设,即其作用是清除感觉淋巴中过量的铵离子。在后者中,RT-PCR和异源表达已用于检查An的表达和功能特征。冈比亚铵转运蛋白AgAmt。我们现在采用先进的转基因工具,全面检查跨幼虫和成年雌性An的外周化学感觉附属物的AgAmt空间定位。冈比亚 在幼虫触角中,AgAmt出现在神经元细胞和辅助细胞中。与D. melanogaster相比,在成年触角中,在非神经元辅助细胞以及氨反应性碱性和腔锥性感觉神经的感觉神经元中都观察到了AgAmt衍生的信号。在上颌触诊,小la和睑裂中,AgAmt似乎仅限于感觉神经元。我们还已经表征了成人触角腔圆锥形感官和上颚掌头状肌钉对氨的反应,揭示了感官AgAmt表达与氨敏感性之间的相关性。综上所述,这些数据表明,AgAmt可能在成虫和幼虫的化学感应装置中发挥不同的作用,并可能在蚊子控制中作为超受体靶标广泛应用。
更新日期:2020-03-02
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