Fumonisins contamination of food commodities is a worldwide problem, especially for maize. The ability to produce fumonisinsis a trait of several species of Fusarium, mainly F. verticillioides and F. proliferatum on maize, and some Aspergillus species. A. niger and its sister species A. welwitschiae, can contribute to fumonisin B2 (FB2) accumulation in maize kernels, although to a lesser extent than fumonisin-producing Fusarium species. Fumonisins risk monitoring represents an effective strategy in the integrated approach for mycotoxin risk management and reduction. The availability of a user-friendlymolecular assay for the detection oftoxigenic fungal species represents a valuable tool in understanding and managing upcoming mycotoxin contamination. In this study, we developed a LAMP assay, based on the detection of fum10, for a rapid and specific molecular detection of FB2-producing A. niger and A. welwistchiae, potentially useful to perform monitoring directly "on site" in maize chain. Results showed that very low amounts of conidia are suitable to detect the presence of the target gene, thus providing information about the presence of FB2-producing Aspergillus species and the possible upcoming fumonisins contamination in maize. The assay was combined with a suitable protocol for "in field" crude DNA extraction and a colorimetric method for easy naked-eye evaluationof results, offering a reliable and user-friendly tool to support effective reduction strategies of mycotoxin contamination in crop management programs.

中文翻译：

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循环介导的等温扩增（LAMP）分析可快速检测产生伏马毒素的曲霉菌种。

伏马毒素对食品的污染是一个世界性的问题，特别是对于玉米而言。产生伏马毒素的能力是玉米上镰刀菌的几种物种的特征，主要是玉米上的拟南芥和拟南芥，以及一些曲霉属种。黑曲霉及其姐妹种A. welwitschiae可以促进玉米粒中伏马毒素B2（FB2）的积累，尽管程度不及产伏马毒素的镰刀菌物种。伏马菌素风险监测是霉菌毒素风险管理和降低的综合方法中的有效策略。用于检测毒素真菌种类的用户友好型分子测定方法的可用性代表了了解和管理即将到来的霉菌毒素污染的宝贵工具。在这项研究中，我们基于对fum10的检测，开发了LAMP测定法，可以快速，特异性地检测生产FB2的黑曲霉和weledistae科，可能直接用于在玉米链中“现场”进行监测。结果表明，极低的分生孢子量适合检测目标基因的存在，从而提供了有关产生FB2的曲霉菌种的存在以及玉米中可能存在的伏马菌素污染的信息。该测定法与适用于“现场”粗DNA提取的合适方案和比色法相结合，可轻松用肉眼评估结果，从而提供了一种可靠且用户友好的工具，可支持作物管理计划中有效的霉菌毒素污染减少策略。