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Morphology and quantification of fungal growth in residential dust and carpets
Building and Environment ( IF 7.1 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.buildenv.2020.106774
Nicholas Nastasi 1, 2, 3 , Sarah R Haines 1, 2, 3 , Lingyi Xu 4 , Hadler da Silva 5 , Adnan Divjan 5 , Mark A Barnes 6 , Chad A Rappleye 7 , Matthew S Perzanowski 5 , Brett J Green 6 , Karen C Dannemiller 1, 2
Affiliation  

Abstract Mold growth indoors is associated with negative human health effects, and this growth is limited by moisture availability. Dust deposited in carpet is an important source of human exposure due to potential elevated resuspension compared to hard floors. However, we need an improved understanding of fungal growth in dust and carpet to better estimate human exposure. The goal of this study was to compare fungal growth quantity and morphology in residential carpet under different environmental conditions, including equilibrium relative humidity (ERH) (50%, 85%, 90%, 95%, 100%), carpet fiber material (nylon, olefin, wool) and presence/absence of dust. We analyzed incubated carpet and dust samples from three Ohio homes for total fungal DNA, fungal allergen Alt a 1, and fungal morphology. Dust presence and elevated ERH (≥85%) were the most important variables that increased fungal growth. Elevated ERH increased mean fungal DNA concentration (P

中文翻译:


住宅灰尘和地毯中真菌生长的形态学和定量



摘要 室内霉菌的生长会对人类健康产生负面影响,并且这种生长受到水分利用率的限制。与硬地板相比,地毯上沉积的灰尘是人体暴露的重要来源,因为其再悬浮可能会增加。然而,我们需要更好地了解灰尘和地毯中的真菌生长,以更好地估计人类接触情况。本研究的目的是比较不同环境条件下住宅地毯的真菌生长数量和形态,包括平衡相对湿度(ERH)(50%、85%、90%、95%、100%)、地毯纤维材料(尼龙) 、烯烃、羊毛)以及是否存在灰尘。我们分析了来自俄亥俄州三个家庭的孵化地毯和灰尘样本的总真菌 DNA、真菌过敏原 Alt a 1 和真菌形态。灰尘的存在和 ERH 升高(≥85%)是增加真菌生长的最重要变量。 ERH 升高会增加平均真菌 DNA 浓度 (P
更新日期:2020-05-01
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