Abstract Bioenergetic analysis implies that microorganisms can harvest the released energy from metal corrosion for survival via extracellular electron transfer (EET) under carbon-starvation conditions. In this work, we combined carbon starvation with CTC/DAPI counterstaining and developed a novel method for detecting EET from metal substrate to the cells. A wild-type nitrate-reducing bacterium (NRB) Bacillus licheniformis was evidenced to be able to take up electrons from the X80 steel substrate for respiration. This study provides reliable support to the bioenergetic theory for microbiologically influenced corrosion (MIC) of carbon steel by NRB.

中文翻译：

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用氧化还原探针、5-氰基-2,3-二甲苯基氯化四唑证明地衣芽孢杆菌从 X80 钢中吸收电子

摘要 生物能分析表明，微生物可以在碳饥饿条件下通过细胞外电子转移（EET）从金属腐蚀中获取释放的能量以求生存。在这项工作中，我们将碳饥饿与 CTC/DAPI 复染相结合，开发了一种检测从金属底物到细胞的 EET 的新方法。一种野生型硝酸盐还原菌 (NRB) 地衣芽孢杆菌被证明能够从 X80 钢基材吸收电子进行呼吸。该研究为 NRB 对碳钢的微生物影响腐蚀 (MIC) 的生物能理论提供了可靠的支持。