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Identification of transcriptional responsive genes to acetic acid, ethanol, and 2-phenylethanol exposure in Drosophila melanogaster
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.02.018 Keon Mook Seong 1 , YeongHo Kim 2 , Donghun Kim 2 , Barry R Pittendrigh 3 , Young Ho Kim 2
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.02.018 Keon Mook Seong 1 , YeongHo Kim 2 , Donghun Kim 2 , Barry R Pittendrigh 3 , Young Ho Kim 2
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The fruit fly, Drosophila melanogaster, is predominantly found in overripe, rotten, fermenting, or decaying fruits and is constantly exposed to chemical stressors such as acetic acid, ethanol, and 2-phenylethanol. D. melanogaster has been employed as a model system for studying the molecular bases of various types of chemical-induced tolerance. Expression profiling using Illumina sequencing has been performed for identifying changes in gene expression that may be associated with evolutionary adaptation to exposure of acetic acid, ethanol, and 2-phenylethanol. We identified a total of 457 differentially expressed genes that may affect sensitivity or tolerance to three chemicals in the chemical treatment group as opposed to the control group. Gene-set enrichment analysis revealed that the genes involved in metabolism, multicellular organism reproduction, olfaction, regulation of signal transduction, and stress tolerance were over-represented in response to chemical exposure. Furthermore, we also detected a coordinated upregulation of genes in the Toll- and Imd-signaling pathways after the chemical exposure. Quantitative reverse transcription PCR analysis revealed that the expression levels of nine genes within the set of genes identified by RNA sequencing were up- or downregulated owing to chemical exposure. Taken together, our data suggest that such differentially expressed genes are coordinately affected by chemical exposure. Transcriptional analyses after exposure of D. melanogaster with three chemicals provide unique insights into subsequent functional studies on the mechanisms underlying the evolutionary adaptation of insect species to environmental chemical stressors.
中文翻译：
在黑腹果蝇中鉴定对乙酸、乙醇和 2-苯乙醇暴露的转录反应基因
果蝇 Drosophila melanogaster 主要存在于过熟、腐烂、发酵或腐烂的水果中，并不断暴露于化学压力源，如醋酸、乙醇和 2-苯基乙醇。D. melanogaster 已被用作研究各种类型化学诱导耐受的分子基础的模型系统。使用 Illumina 测序的表达谱已用于鉴定基因表达的变化，这些变化可能与进化适应暴露于乙酸、乙醇和 2-苯基乙醇有关。我们总共鉴定了 457 个差异表达的基因，这些基因可能会影响化学处理组而不是对照组对三种化学物质的敏感性或耐受性。基因集富集分析表明，参与代谢的基因，多细胞生物的繁殖、嗅觉、信号转导的调节和应激耐受性在对化学物质暴露的反应中过多。此外，我们还检测到化学暴露后 Toll 和 Imd 信号通路中基因的协调上调。定量逆转录 PCR 分析表明，RNA 测序鉴定的一组基因中的九个基因的表达水平由于化学暴露而上调或下调。总之，我们的数据表明这种差异表达的基因受到化学暴露的协同影响。暴露 D 后的转录分析。
更新日期：2020-05-01
中文翻译：
在黑腹果蝇中鉴定对乙酸、乙醇和 2-苯乙醇暴露的转录反应基因
果蝇 Drosophila melanogaster 主要存在于过熟、腐烂、发酵或腐烂的水果中，并不断暴露于化学压力源，如醋酸、乙醇和 2-苯基乙醇。D. melanogaster 已被用作研究各种类型化学诱导耐受的分子基础的模型系统。使用 Illumina 测序的表达谱已用于鉴定基因表达的变化，这些变化可能与进化适应暴露于乙酸、乙醇和 2-苯基乙醇有关。我们总共鉴定了 457 个差异表达的基因，这些基因可能会影响化学处理组而不是对照组对三种化学物质的敏感性或耐受性。基因集富集分析表明，参与代谢的基因，多细胞生物的繁殖、嗅觉、信号转导的调节和应激耐受性在对化学物质暴露的反应中过多。此外，我们还检测到化学暴露后 Toll 和 Imd 信号通路中基因的协调上调。定量逆转录 PCR 分析表明，RNA 测序鉴定的一组基因中的九个基因的表达水平由于化学暴露而上调或下调。总之，我们的数据表明这种差异表达的基因受到化学暴露的协同影响。暴露 D 后的转录分析。
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