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The composition of phospholipid model bacterial membranes determines their endurance to secretory phospholipase A2 attack - The role of cardiolipin.
Biochimica et Biophysica Acta (BBA) - Biomembranes ( IF 2.8 ) Pub Date : 2020-02-28 , DOI: 10.1016/j.bbamem.2020.183239 Paulina Perczyk 1 , Aneta Wójcik 1 , Natalia Hachlica 1 , Paweł Wydro 2 , Marcin Broniatowski 1
Biochimica et Biophysica Acta (BBA) - Biomembranes ( IF 2.8 ) Pub Date : 2020-02-28 , DOI: 10.1016/j.bbamem.2020.183239 Paulina Perczyk 1 , Aneta Wójcik 1 , Natalia Hachlica 1 , Paweł Wydro 2 , Marcin Broniatowski 1
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Soil bacteria are decomposer organisms crucial for the biodegradation of organic pollutants, mineralization of dead organic matter and the turnover of biogenic elements. In their environment they are constantly exposed to membrane-lytic enzymes emitted to the soil by other microorganisms competing for the same niche. Therefore, the composition and structure of their membranes is of utmost importance for survival in the harsh environment. Although soil bacteria species can be Gram-negative or Gram-positive and their membranes differ significantly, they are formed by phospholipids belonging mainly to three classes: phosphatidylethanolamines (PE), phosphatidylglycerols (PG) and cardiolipins (CL). The correlation of the membrane phospholipid composition and its susceptibility to secretory membrane-lytic enzymes is widely unknown; thus, to shed light on these phenomena we applied the Langmuir monolayer technique to construct models of soil bacteria membranes differing in the mutual proportion of the main phospholipids. To characterize the systems we studied their elasticity, mesoscopic texture, 2D crystalline structure and discussed the thermodynamics of the interactions between their components. The model membranes were exposed to secretory phospholipase A2. It turned out that in spite of the structural similarities the model membranes differed significantly in their susceptibility to s-PLA2 attack. The membranes devoid of cardiolipin were completely degraded, whereas, these containing cardiolipin were much more resistant to the enzymatic hydrolysis. It also turned out that the sole presence of cardiolipin in the model membrane did not guarantee the membrane durability and that the interplay between cardiolipin and the zwitterionic phosphatidylethanolamine was here of crucial importance.
中文翻译：
磷脂模型细菌膜的组成决定了它们对分泌性磷脂酶A2攻击的耐受性-心磷脂的作用。
土壤细菌是分解有机物，对有机污染物的生物降解，死有机物的矿化和生物元素的转换至关重要。在他们的环境中，它们不断暴露于其他微生物竞争同一生态位而释放到土壤中的膜分解酶。因此，其膜的组成和结构对于在恶劣环境下的生存至关重要。尽管土壤细菌种类可以是革兰氏阴性或革兰氏阳性，并且它们的膜明显不同，但它们是由主要属于三类的磷脂形成的：磷脂酰乙醇胺（PE），磷脂酰甘油（PG）和心磷脂（CL）。膜磷脂组成及其对分泌性膜分解酶敏感性的相关性尚不清楚。从而，为了阐明这些现象，我们应用Langmuir单层技术构建了主要磷脂相互比例不同的土壤细菌膜模型。为了表征系统，我们研究了它们的弹性，介观织构，2D晶体结构，并讨论了它们之间相互作用的热力学。使模型膜暴露于分泌性磷脂酶A2。结果表明，尽管结构上相似，但模型膜对s-PLA2攻击的敏感性却存在显着差异。不含心磷脂的膜被完全降解，而含有心磷脂的膜对酶水解的抵抗力强得多。
更新日期：2020-03-02
中文翻译：
磷脂模型细菌膜的组成决定了它们对分泌性磷脂酶A2攻击的耐受性-心磷脂的作用。
土壤细菌是分解有机物，对有机污染物的生物降解，死有机物的矿化和生物元素的转换至关重要。在他们的环境中，它们不断暴露于其他微生物竞争同一生态位而释放到土壤中的膜分解酶。因此，其膜的组成和结构对于在恶劣环境下的生存至关重要。尽管土壤细菌种类可以是革兰氏阴性或革兰氏阳性，并且它们的膜明显不同，但它们是由主要属于三类的磷脂形成的：磷脂酰乙醇胺（PE），磷脂酰甘油（PG）和心磷脂（CL）。膜磷脂组成及其对分泌性膜分解酶敏感性的相关性尚不清楚。从而，为了阐明这些现象，我们应用Langmuir单层技术构建了主要磷脂相互比例不同的土壤细菌膜模型。为了表征系统，我们研究了它们的弹性，介观织构，2D晶体结构，并讨论了它们之间相互作用的热力学。使模型膜暴露于分泌性磷脂酶A2。结果表明，尽管结构上相似，但模型膜对s-PLA2攻击的敏感性却存在显着差异。不含心磷脂的膜被完全降解，而含有心磷脂的膜对酶水解的抵抗力强得多。
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