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Zebrafish miR-462-731 is required for digestive organ development.
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2020-02-29 , DOI: 10.1016/j.cbd.2020.100679 Yan Huang 1 , Chun-Xiao Huang 1 , Wei-Feng Wang 1 , Hong Liu 1 , Huan-Ling Wang 1
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2020-02-29 , DOI: 10.1016/j.cbd.2020.100679 Yan Huang 1 , Chun-Xiao Huang 1 , Wei-Feng Wang 1 , Hong Liu 1 , Huan-Ling Wang 1
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MicroRNAs (miRNAs), as important regulators of post-transcriptional gene expression, play important roles in the occurrence and function of organs. In this study, morpholino (MO) knockdown of miR-462/miR-731 was used to investigate the potential mechanisms of the miR-462-731 cluster during zebrafish liver development. The results showed significant reduction of digestive organs, especially liver and exocrine pancreas after the miR-462/miR-731 knockdown, and those phenotypes could be partially rescued by corresponding miRNA duplex. Acinar cells of the exocrine pancreas were also severely affected with pancreatic insufficiency. In particular, knockdown of miR-462 caused pancreas morphogenesis abnormity with specific bilateral exocrine pancreas. Additionally, it was found that miR-731 played a role in liver and exocrine pancreas development by directly targeting dkk3b, instead of the down-regulation of Wnt/β-catenin signaling. These findings contribute significantly to our understanding of molecular mechanisms of miR-462-731 cluster in development of digestive organs.
中文翻译：
斑马鱼miR-462-731是消化器官发育所必需的。
微小RNA（miRNA）作为转录后基因表达的重要调节剂，在器官的发生和功能中起重要作用。在这项研究中，使用miR-462 / miR-731的吗啉代（MO）敲低来研究miR-462-731集群在斑马鱼肝脏发育过程中的潜在机制。结果显示，miR-462 / miR-731敲低后，消化器官特别是肝脏和外分泌胰腺显着减少，并且相应的miRNA双链体可以部分挽救这些表型。外分泌胰腺的腺泡细胞也受到胰腺功能不全的严重影响。特别是，敲低miR-462会导致特定双侧外分泌胰腺胰腺形态发生异常。另外，已经发现，miR-731通过直接靶向dkk3b而不是下调Wnt /β-catenin信号传导，在肝脏和外分泌胰腺的发育中起作用。这些发现大大有助于我们了解miR-462-731簇在消化器官发育中的分子机制。
更新日期：2020-03-02
中文翻译：
斑马鱼miR-462-731是消化器官发育所必需的。
微小RNA（miRNA）作为转录后基因表达的重要调节剂，在器官的发生和功能中起重要作用。在这项研究中，使用miR-462 / miR-731的吗啉代（MO）敲低来研究miR-462-731集群在斑马鱼肝脏发育过程中的潜在机制。结果显示，miR-462 / miR-731敲低后，消化器官特别是肝脏和外分泌胰腺显着减少，并且相应的miRNA双链体可以部分挽救这些表型。外分泌胰腺的腺泡细胞也受到胰腺功能不全的严重影响。特别是，敲低miR-462会导致特定双侧外分泌胰腺胰腺形态发生异常。另外，已经发现，miR-731通过直接靶向dkk3b而不是下调Wnt /β-catenin信号传导，在肝脏和外分泌胰腺的发育中起作用。这些发现大大有助于我们了解miR-462-731簇在消化器官发育中的分子机制。
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