Abstract The single-use stirred bioreactors are increasingly recognized as a viable alternative in animal cell culture due to the higher production capacity, increased flexibility, prevention of cross contamination, reduction of the cleaning cost, and shortened downtime. In this paper, the effects of the impeller speed, the volumetric gas flow rate, and the impeller type on the volumetric gas-liquid mass transfer coefficient in a single-use unbaffled angled-shaft bioreactor applicable for the animal cell culture were analyzed and compared with those attained for the baffled vertical-shaft bioreactors. The volumetric gas-liquid mass transfer coefficient (KLa) was experimentally determined by the simplified dynamic pressure method. The lowest P/V values as well as the highest KLa values were achieved for an unbaffled angled-shaft bioreactor. It was found that the Rushton impeller was the most efficient impeller in term of mass transfer for this bioreactor. An empirical correlation for the mass transfer coefficient was also developed.

中文翻译：

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适用于动物细胞培养的一次性斜轴曝气搅拌生物反应器中的传质

摘要 一次性搅拌生物反应器由于具有更高的生产能力、更高的灵活性、防止交叉污染、降低清洁成本和缩短停机时间，因此越来越被认为是动物细胞培养的可行替代方案。本文分析比较了适用于动物细胞培养的一次性无挡板斜轴生物反应器中叶轮转速、气体体积流量和叶轮类型对体积气液传质系数的影响。与挡板垂直轴生物反应器所获得的结果相同。体积气液传质系数 (KLa) 是通过简化的动压法通过实验确定的。对于无挡板的斜轴生物反应器，实现了最低的 P/V 值和最高的 KLa 值。结果发现，就该生物反应器的传质而言，Rushton 叶轮是最有效的叶轮。还开发了传质系数的经验相关性。