Spiroacetals are widespread in nature as components of volatile semiochemical secretions from many insect species. The general pathway for spiroacetal biosynthesis in Bactrocera sp. is preliminarily established, but many genes involved in this pathway remain to be characterized. By analyzing transcriptomes of the rectal glands (RGs) from immature and mature females of the oriental fruit fly, Bactrocera dorsalis, a set of genes encoding two acetyl-CoA carboxylases (ACCs), two fatty acid synthases (FASs), eight desaturases (DESs), twelve fatty acyl-CoA reductases (FARs), seventy-two cytochrome P450 enzymes (CYPs), and twenty-three odorant binding proteins (OBPs) were identified. We investigated the expression of candidate genes in immature and mature stages based on the RNA-seq data and Real-time quantitative PCR. Expression profiling revealed that some of these genes were primarily expressed in female rectal glands among different tissues, and were up-regulated in mature females. Semi-quantitative RT-PCR assays were also adapted to examine tissue-specific expression of selected candidate genes. Additionally, their putative functions in spiroacetal synthesis and transportation are proposed. Our study provided large-scale sequence information for further functional studies on spiroacetal biosynthetic pathways.

螺缩醛作为来自许多昆虫物种的挥发性信息化学分泌物的成分在自然界中广泛存在。Bactrocera sp。中螺乙醛生物合成的一般途径。虽然已经初步建立了RNAi，但是该途径中涉及的许多基因仍有待表征。通过分析从不成熟的直肠腺（RGS）的转录和成熟的桔小实蝇的女性，桔小实蝇，一组编码两种乙酰辅酶A羧化酶（ACC），两种脂肪酸合酶（FAS），八种去饱和酶（DES），十二种脂肪酰基辅酶A还原酶（FAR），七十二种细胞色素P450酶（CYP）的基因，以及鉴定了二十三种气味结合蛋白（OBP）。我们基于RNA-seq数据和实时定量PCR研究了未成熟阶段和成熟阶段候选基因的表达。表达谱分析表明，其中一些基因主要在不同组织之间的女性直肠腺体中表达，并在成熟女性中被上调。半定量RT-PCR测定法也适用于检查所选候选基因的组织特异性表达。另外，提出了它们在螺缩醛合成和运输中的假定功能。