Oxidative stress reflects the imbalance of pro-oxidants and antioxidants. Prolonged oxidative stress can induce cellular damage, diseases and aging, and the effects may be sex-specific. Tigriopus californicus has recently been proposed as an alternative model system for sex-specific studies due to the absence of sex chromosomes. In this study, we used comparative transcriptomic analyses to assess sex-specific transcriptional responses to oxidative stress. Male and female individuals were maintained separately in one of three treatments: 1) control conditions with an algae diet, 2) pro-oxidant (H₂O₂) conditions with an algae diet or 3) decreased antioxidant conditions (reduced carotenoids due to a yeast diet). Single individual RNA-seq was then conducted for twenty-four libraries using Ligation Mediated RNA sequencing (LM-Seq). Variance in gene expression was partitioned into 62.3% between sexes, 26.85% among individuals and 10.85% among treatments. Within each of the three treatments, expression was biased toward females. However, compared to the control treatment, males in both pro-oxidant and decreased antioxidant treatments differentially expressed more genes while females differentially expressed fewer genes but with a greater magnitude of fold change. As the first study of copepods to apply single individual RNA-seq, the findings will contribute to a better understanding of transcriptomic variation among individuals as well as sex-specific response mechanisms to oxidative stress in the absence of sex chromosomes.

氧化应激反映了前氧化剂和抗氧化剂的不平衡。长时间的氧化应激会引起细胞损伤，疾病和衰老，其影响可能是性别特异性的。由于不存在性染色体，最近已提出将Tigriopus californicus用作性别特定研究的替代模型系统。在这项研究中，我们使用了比较转录组学分析来评估针对氧化应激的性别特异性转录反应。男性和女性个体分别接受以下三种治疗之一：1）用藻类饮食控制病况； 2）前氧化剂（H ₂ O _2））使用藻类饮食的情况或3）抗氧化剂条件降低（由于酵母饮食而减少的类胡萝卜素）。然后使用连接介导的RNA测序（LM-Seq）对24个文库进行单个RNA测序。基因表达的差异被划分为性别之间的62.3％，个体之间的26.85％和治疗之间的10.85％。在这三种治疗方法的每一种中，表达都偏向女性。然而，与对照治疗相比，在抗氧化剂和抗氧化剂减少的雄性中差异表达更多的基因，而雌性中差异表达更少的基因，但是倍数变化更大。作为对pe足类动物的第一个研究，它应用单个单独的RNA-seq，