Neuropeptide Y (NPY) receptors are suggested to mediate the multi-physiological functions of NPY family peptides, such as food intake, in teleost fish. However, the structure and signaling of fish NPY receptors are yet to be fully elucidated. In this study, we report the cloning and characterization of two neuropeptide Y receptor subtypes, Y2 (NPY2R) and Y7 (NPY7R), in yellow croaker Larimichthys crocea (L. crocea) (LcNPY2R, LcNPY7R). The gene structure, pharmacological characterization, cell location, and tissue expression of these two receptors were explored. The phylogenetic results showed that LcNPY2R and LcNPY7R had typical G protein-coupled receptor profiles, associated with the Y2 subfamily, with coding sequences that are highly conserved in vertebrates. The expression of both LcNPY2R and LcNPY7R could be activated by LcNPY in HEK293 cells. However, truncated LcNPY_18–36 was only able to activate LcNPY2R at the same level as full length LcNPY. Expression analysis revealed that LcNPY2R mRNA was predominantly expressed in the intestine and liver, whereas LcNPY7R was expressed in the stomach, which indicated that both receptors were related to the digestive system. Overall, our data establishes a molecular basis to determine the actions of LcNPY2R and LcNPY7R, which could be used to elucidate the conserved roles of these receptor-ligand pairs in vertebrates.

建议神经肽Y（NPY）受体介导硬骨鱼中NPY家族肽的多种生理功能，例如食物摄入。然而，鱼NPY受体的结构和信号转导尚待充分阐明。在这项研究中，我们报告两个神经肽Y受体亚型的克隆和鉴定，Y2（NPY2R）和Y7（NPY7R），在黄鱼大黄鱼（L.大黄鱼）（LC NPY2R，LC NPY7R） 。研究了这两种受体的基因结构，药理学特征，细胞位置和组织表达。系统发育结果表明Lc NPY2R和LcNPY7R具有典型的G蛋白偶联受体谱，与Y2亚科相关，编码序列在脊椎动物中高度保守。两者的表达LC NPY2R和LC NPY7R可以通过激活LC NPY在HEK293细胞中。但是，截短的Lc NPY _{18 – 36}只能以与全长Lc NPY相同的水平激活Lc NPY2R 。表达分析表明，Lc NPY2R mRNA主要在肠和肝中表达，而LcNPY7R在胃中表达，表明这两种受体都与消化系统有关。总体而言，我们的数据为确定Lc NPY2R和Lc NPY7R的作用奠定了分子基础，可用于阐明这些受体-配体对在脊椎动物中的保守作用。