Insulin-like growth factors (Igf1 and Igf2) play a key role in growth and development of vertebrates. In mammals, the expression of IGFs is regulated by estradiol-17β (E₂) via estrogen receptors (ESRs). The expression of igfs can also be regulated by E₂ in fish, while comparative study of this is still lacking. The present study examined tissue distribution of igfs and hepatic expression of igfs and esrs during gonad development in Scatophagus argus by real-time PCR. Serum E₂ concentration was measured by enzyme-linked immunosorbent assay (ELISA). The hepatic expression of igfs and esrs at gonadal phase III, incubated with either E₂ (0.1, 1 or 10 μM) alone or in combination with estrogen receptor antagonists-fulvestrant, MPP or PHTPP, was measured. igf1 and igf2 expressed highest in liver of both sexes. Igf1, esr1 and esr2b expressions and serum E₂ concentration increased, while igf2 and esr2a expressions decreased, during ovary development. Igfs and esrs expressions increased while serum E₂ concentration maintained low during testis development. In females, E₂ incubation enhanced the expressions of igf1 and esr1 but inhibited that of igf2 and esr2a. Both fulvestrant and MPP inhibited up-regulation effect of E₂ on igf1 and esr1. Fulvestrant enhanced down-regulation effect of E₂ on igf2 and esr2a, but MPP conversely. In males, E₂ incubation enhanced the expressions of igfs, esr1 and esr2a. Fulvestrant and MPP inhibited up-regulation effect of E₂ on igfs and esr1. PHTPP inhibited igf1 and esr2 expressions in both sexes. Our results indicated that the expression of igfs is regulated by E₂ via Esrs in S. argus.

胰岛素样生长因子（Igf1和Igf2）在脊椎动物的生长发育中起关键作用。在哺乳动物中，IGF s的表达受雌激素受体（ESRs）的雌二醇17β（E ₂）调节。igf s的表达也可以通过鱼类中的E ₂来调节，但尚缺乏对此的比较研究。本研究检查的组织中的分布的IGFs和的肝表达IGF S和ESR在性腺发育在S金钱鱼通过实时PCR。通过酶联免疫吸附测定（ELISA）测量血清E ₂浓度。igf的肝表达测量了性腺阶段III中的s和esr，分别与E ₂（0.1、1或10μM）或与雌激素受体拮抗剂-氟维司群，MPP或PHTPP组合孵育。igf1和igf2在男女肝脏中表达最高。在卵巢发育过程中，Igf1，esr1和esr2b表达和血清E ₂浓度升高，而igf2和esr2a表达降低。在睾丸发育过程中，Igf和esr的表达增加，而血清E _2的浓度保持较低。在女性中，E ₂孵育增强了igf1和esr1的表达，但抑制了igf2和esr2a的表达。氟维司群和MPP均抑制E ₂对igf1和esr1的上调作用。溶剂型增强了E ₂对igf2和esr2a的下调作用，但MPP相反。在雄性中，E ₂孵育增强的表达IGF S，ESR1和esr2a。氟维司群和MPP抑制上调E对₂对IGF S和ESR1。PHTPP抑制男女的igf1和esr2表达。我们的研究结果表明，表达胰岛素样生长因子由E调节₂经由ESRS在S. ARGUS。