L-asparaginase (L-ASNase) is the gold standard enzyme used to treat acute lymphoblastic leukemia. This disease primarily affects children; however, treatment increases survival from 20% to 90%. As a bioproduct, it is obtained via a biotechnological process and its purification usually consists of several steps that account for up to 80% of the total production costs. This review discusses available strategies for the purification of L-ASNase and highlights a process with fewer steps, and consequently, lower cost and higher yield. This process emphasizes the possibility of using a novel aqueous two-phase system extraction process to purify L-ASNase.

L-天冬酰胺酶（L-ASNase）是用于治疗急性淋巴细胞白血病的金标准酶。这种疾病主要影响儿童。但是，治疗可以将生存率从20％提高到90％。作为生物产品，它是通过生物技术过程获得的，其提纯通常包括几个步骤，这些步骤最多占总生产成本的80％。这篇评论讨论了纯化L-ASNase的可用策略，并着重介绍了步骤更少，因此成本更低，产量更高的工艺。该过程强调了使用新颖的水相两相系统萃取过程纯化L-ASNase的可能性。