Polyphenols are secondary plant metabolites playing a major role as potentially functional components. They can also be used for honey authentication. This review gathers the recent literature references about honey extraction procedures, as well as instrumental analysis of phenolic compounds found in honey. Liquid-Liquid extraction is widely used for both extraction and purification purposes, with adequate recovery percentages. However, the use of high solvent volumes is a major disadvantage. More environmentally friendly methods include accelerated solvent extraction, and dispersive and inverse dispersive liquid-liquid microextraction. Solid phase extraction is the most common method for honey polyphenols’ isolation. Polyphenol isolation by a combination of liquid-liquid and solid phase extraction allows good recoveries for a variety of different compounds. High-performance liquid chromatography with ultraviolet or mass spectrometry detectors is by far, the most commonly employed instrumental procedure to separate and quantify polyphenols in honey although capillary electrophoresis has been also successfully used for these purposes. The use of new sorbents, the optimization of current procedures and the development of other simple and rapid analytical techniques are challenges for future analysis of polyphenols found in honey.

多酚是植物的次生代谢产物，主要起潜在的功能成分作用。它们也可以用于蜂蜜认证。这篇综述收集了有关蜂蜜提取程序以及对蜂蜜中发现的酚类化合物进行仪器分析的最新文献参考。液-液萃取被广泛用于萃取和纯化目的，并具有足够的回收率。然而，使用高溶剂体积是主要缺点。更环保的方法包括加速溶剂萃取，分散和逆分散液-液微萃取。固相萃取是分离蜂蜜多酚的最常用方法。通过液-液相和固相萃取的组合进行多酚分离，可以对多种不同的化合物进行良好的回收。迄今为止，具有紫外或质谱检测器的高效液相色谱法是分离和定量蜂蜜中多酚的最常用的仪器程序，尽管毛细管电泳也已成功用于这些目的。新吸附剂的使用，当前程序的优化以及其他简单，快速的分析技术的发展，是未来对蜂蜜中多酚进行分析的挑战。尽管毛细管电泳也已成功用于这些目的，但仍是最常用的仪器程序，用于分离和定量蜂蜜中的多酚。新吸附剂的使用，当前程序的优化以及其他简单，快速的分析技术的发展，是未来对蜂蜜中多酚进行分析的挑战。尽管毛细管电泳也已成功用于这些目的，但仍是最常用的仪器程序，用于分离和定量蜂蜜中的多酚。新吸附剂的使用，当前程序的优化以及其他简单，快速的分析技术的发展，是未来对蜂蜜中多酚进行分析的挑战。