当前位置: X-MOL 学术Sep. Purif. Rev. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Back to BAC: Insights into Boronate Affinity Chromatography Interaction Mechanisms
Separation and Purification Reviews ( IF 5.2 ) Pub Date : 2017-09-25 , DOI: 10.1080/15422119.2017.1365085
Maria Betzabeth Espina-Benitez 1 , Jérôme Randon 1 , Claire Demesmay 1 , Vincent Dugas 1
Affiliation  

Boronate affinity chromatography (BAC) is commonly used in sample preparation techniques for its specific selectivity for cis-diol-containing compounds. Various chromatographic materials based on phenyl-boronic acid (PBA) functionalization have been designed. This review aims to present the versatility of the underlying BAC mechanisms. The PBA moiety presents secondary/complementary/interfering interactions, i.e., hydrophobic, electrostatic and/or hydrogen-bonding interactions. The comprehensive understandings of these mechanisms are very helpful to manipulate separation selectivity, which is a key feature in biomolecular sample treatment. Each BAC interaction is reviewed and illustrated by specific examples. Special attention is given to the underlying role of PBA supports for sugars, glycans and glycoconjugates recognition.



中文翻译:

返回BAC:硼酸亲和色谱相互作用机理的见解

硼酸酯亲和色谱法(BAC)因其对含顺式二醇的化合物的特异性选择性而常用于样品制备技术中。已经设计了基于苯基硼酸(PBA)官能化的各种色谱材料。这篇综述旨在介绍基础BAC机制的多功能性。PBA部分呈现次级/互补/干扰相互作用,即疏水,静电和/或氢键相互作用。对这些机制的全面理解对于操纵分离选择性非常有帮助,这是生物分子样品处理的关键特征。每种BAC交互都将通过特定示例进行回顾和说明。特别注意PBA支持对糖,聚糖和糖缀合物识别的潜在作用。

更新日期:2017-09-25
down
wechat
bug