Research on artemisinin and its derivatives (ARTDs) were initiated due to their outstanding anti-malarial effects. In the past few decades, considerable efforts have been made to extend the clinical applications of ARTDs. However, some ARTDs have a low aqueous solubility, short half-life in vivo and instability due to an endoperoxide bridge in their structures that also contributes to a poor bioavailability. The development of efficient quantitative analysis methods is needed for many branches of research, such as the chemical compound determination in plants, drug quality control and drug metabolism studies. The analysis methods used to detect ARTDs include spectroscopy, liquid chromatography, mass spectrometry and immunoassays. However, a systematic quantitative analysis of these compounds as the very first step in many studies has not been always conducted. Thus, this review presents a comprehensive overview of available analytical methods for in vivo and in vitro quantitation of ARTDs. It generalizes and compares the various methods focusing on sample preparation.

由于青蒿素及其衍生物具有出色的抗疟疾作用，因此开始进行研究。在过去的几十年中，为扩展ARTD的临床应用做出了巨大的努力。然而，一些ARTDs的水溶性低，体内半衰期短并且由于其结构中的过氧化物过氧化物桥而不稳定，这也导致不良的生物利用度。许多研究领域都需要开发有效的定量分析方法，例如植物中化合物的确定，药物质量控制和药物代谢研究。用于检测ARTD的分析方法包括光谱法，液相色谱法，质谱法和免疫测定法。然而，作为许多研究的第一步，并不总是对这些化合物进行系统的定量分析。因此，本综述全面概述了可用的体内和体外ARTD定量分析方法。它归纳并比较了侧重于样品制备的各种方法。