Abstract A commercially available lactic acid bacteria (LAB) reference standard (EZ-Accu ShotTM Lactobacillus rhamnosus) was evaluated for its suitability to be used in the microbiological proficiency test (PT) schemes that are utilized within brewery microbiological quality systems and protocols. The test material was evaluated for homogeneity, stability, and culturability on several routinely used beer-spoiler detection media using membrane filtration (MF). Mean numbers of CFUs were calculated and subjected to statistical analysis. The coefficient of variation (CV) value in the homogeneity test (10.6%) showed that the test material was sufficiently homogenous, and the pellet-to-pellet variation was acceptable. The P-value in the stability and culturability test (p > 0.05) showed no statistically significant differences between variables, indicating that the microorganisms in the pellet would be stable during long storage and transport and that the culturability on different beer-spoiler detection media are comparable. In conclusion, this study suggests that EZ-Accu ShotTM L. rhamnosus is suitable as a test material for microbiological PT schemes in breweries.

中文翻译：

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评估微生物参考标准 (EZ-Accu ShotTM) 作为啤酒厂能力测试 (PT) 计划的测试材料

摘要 评估了市售乳酸菌 (LAB) 参考标准品 (EZ-Accu ShotTM 鼠李糖乳杆菌) 是否适用于啤酒厂微生物质量系统和协议中使用的微生物能力测试 (PT) 计划。使用膜过滤 (MF) 在几种常规使用的啤酒扰流器检测介质上评估测试材料的均匀性、稳定性和可培养性。计算 CFU 的平均数并进行统计分析。均质性试验中的变异系数（CV）值（10.6%）表明试验材料具有足够的均质性，颗粒与颗粒之间的差异是可以接受的。稳定性和可培养性测试中的 P 值 (p > 0.05) 显示变量之间没有统计学显着差异，表明颗粒中的微生物在长期储存和运输过程中是稳定的，并且在不同的啤酒扰流器检测介质上的可培养性是可比的。总之，这项研究表明 EZ-Accu ShotTM 鼠李糖乳杆菌适合作为啤酒厂微生物 PT 方案的测试材料。