A strategy to incorporate and release the amphiphilic drugs of doxorubicin (DOX) and ibuprofen (IBU) in the same microcapsules is introduced, A layer-by-layer (LbL) assembly of microcapsules with doxorubicin hydrochloride (DOX) or green fluorescent agent, hydrophilic fluorescein isothiocyanate (FITC), encapsulated in CaCO₃ microparticle templates, was conducted via alternatively depositing sodium carboxymethyl cellulose (CMC) and chitosan (CHI) onto IBU or red fluorescent agent (hydrophobic Nile Red) preloaded poly-L-lactide (PLLA) coated magnetic Fe₃O₄-DOX-loaded CaCO₃ (or FITC-loaded) templates. The structure, morphology, composition, magnetic properties and drugs distribution of the obtained microcapsules were characterized by nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), zeta potential analysis, thermogravimetric analysis (TGA), vibrating sample magnetometry (VSM) and confocal laser scanning microscopy. The fluorescent agents loading of FITC and Nile Red were confirmed by observations using confocal laser scanning microscopy. Fluorescence observations showed that the DOX was distributed both in the walls and in the cavities of the microcapsules, while IBU was present in the capsule wall. The in–vitro release of the dual drugs, DOX and IBU, from the microcapsules with different numbers of CHI and CMC layers was characterized. A tunable amount of drug release was achieved by changing the number of layers. The release study indicated that the LBL microcapsules exhibited better sustained release capacity compared to the uncoated microcapsules. The microcapsules inherited a strong magnetic property from the Fe₃O₄ nanoparticles, sufficient for targeting and magnetic hyperthermia drug delivery systems.

引入了将阿霉素（DOX）和布洛芬（IBU）的两亲药物合并并释放到同一微囊中的策略，微囊与盐酸阿霉素（DOX）或绿色荧光剂的亲水性逐层（LbL）组装封装在CaCO ₃微粒模板中的异硫氰酸荧光素（FITC）通过将羧甲基纤维素钠（CMC）和壳聚糖（CHI）交替沉积到IBU或预涂有聚L-丙交酯（PLLA）的红色荧光剂（疏水性尼罗红）上进行磁性Fe ₃ O ₄ -DOX负载的CaCO ₃（或FITC加载）模板。通过核磁共振（NMR），扫描电子显微镜（SEM），ζ电势分析，热重分析（TGA），振动样品磁强分析（VSM）和微电子显微镜对所得微胶囊的结构，形态，组成，磁性和药物分布进行了表征。共聚焦激光扫描显微镜。通过使用共聚焦激光扫描显微镜观察，确认了FITC和尼罗红的荧光剂含量。荧光观察表明，DOX既分布在微囊的壁中又分布在微囊的腔中，而IBU则存在于囊壁中。表征了双药DOX和IBU从具有不同CHI和CMC层数的微胶囊中的体外释放。通过改变层数实现了可调节的药物释放量。释放研究表明，与未包被的微胶囊相比，LBL微胶囊具有更好的持续释放能力。微胶囊继承了铁的强磁性₃ O ₄纳米粒子，足以用于靶向和磁热疗释药系统。