Synthesis of Injectable Shear-Thinning Biomaterials of Various Compositions of Gelatin and Synthetic Silicate Nanoplatelet.,Biotechnology Journal

当前位置： X-MOL 学术 › Biotechnol. J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Synthesis of Injectable Shear-Thinning Biomaterials of Various Compositions of Gelatin and Synthetic Silicate Nanoplatelet.
Biotechnology Journal ( IF 4.7 ) Pub Date : 2020-02-27 , DOI: 10.1002/biot.201900456
Chengbin Xue _{1,

2,

3,

4} , Huifang Xie _{1,

2,

5} , James Eichenbaum _{1,

2} , Yi Chen _{1,

2,

6} , Yonggang Wang _{1,

2,

7} , Floor W van den Dolder _{1,

2,

8,

9} , Junmin Lee _{1,

2} , KangJu Lee _{1,

2} , Shiming Zhang _{1,

2} , Wujin Sun _{1,

2} , Amir Sheikhi _{1,

2,

10,

11} , Samad Ahadian _{1,

2} , Nureddin Ashammakhi _{1,

2,

12} , Mehmet R Dokmeci _{1,

2,

12} , Han-Jun Kim _{1,

2} , Ali Khademhosseini _{1,

2,

12,

13}

Affiliation

Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Key Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, P. R. China.
Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, P. R. China.
Carbohydrate Laboratory, School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, P. R. China.
Beijing Biosis Healing Biological Technology Co., Ltd, Beijing, 102600, P. R. China.
Guangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Division of Heart and Lungs, Department of Cardiothoracic Surgery, University Medical Center Utrecht, Utrecht, GA, 3508, The Netherlands.
Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, CT, 3584, The Netherlands.
Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
Department of Radiological Sciences, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, 90095, USA.
Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, CA, 90095, USA.

Injectable shear‐thinning biomaterials (iSTBs) have great potential for in situ tissue regeneration through minimally invasive therapeutics. Previously, an iSTB was developed by combining gelatin with synthetic silicate nanoplatelets (SNPs) for potential application to hemostasis and endovascular embolization. Hence, iSTBs are synthesized by varying compositions of gelatin and SNPs to navigate their material, mechanical, rheological, and bioactive properties. All compositions (each component percentage; 1.5–4.5%/total solid ranges; 3–9%) tested are injectable through both 5 Fr general catheter and 2.4 Fr microcatheter by manual pressure. In the results, an increase in gelatin contents causes decrease in swellability, increase in freeze‐dried hydrogel scaffold porosity, increase in degradability and injection force during iSTB fabrication. Meanwhile, the amount of SNPs in composite hydrogels is mainly required to decrease degradability and increase shear thinning properties of iSTB. Finally, in vitro and in vivo biocompatibility tests show that the 1.5–4.5% range gelatin–SNP iSTBs are not toxic to the cells and animals. All results demonstrate that the iSTB can be modulated with specific properties for unmet clinical needs. Understanding of mechanical and biological consequences of the changing gelatin–SNP ratios through this study will shed light on the biomedical applications of iSTB on specific diseases.

中文翻译：

明胶和合成硅酸盐纳米片的各种组成的可注射剪切变稀生物材料的合成。

可注射剪切变稀的生物材料（iSTB）具有通过微创疗法进行原位组织再生的巨大潜力。以前，iSTB是通过将明胶与合成硅酸盐纳米血小板（SNP）结合而开发的，有可能应用于止血和血管内栓塞。因此，通过改变明胶和SNP的成分来合成iSTB，以控制其材料，机械，流变学和生物活性特性。测试的所有组合物（每个成分百分比； 1.5-4.5％/总固体含量； 3-9％）都可以通过手动压力通过5 Fr普通导管和2.4 Fr微导管注射。结果，明胶含量的增加会导致iSTB制备过程中的溶胀性降低，冻干水凝胶支架孔隙率增加，降解性和注入力增加。同时，主要要求复合水凝胶中SNP的含量降低iSTB的降解性并增加其剪切稀化性能。最后，体外和体内生物相容性测试显示，明胶-SNP iSTB的1.5-4.5％范围对细胞和动物无毒。所有结果表明，iSTB可以通过未满足临床需求的特定特性进行调节。通过这项研究了解改变明胶-SNP比值对机械和生物学的影响，将为iSTB在特定疾病上的生物医学应用提供启示。所有结果表明，iSTB可以通过未满足临床需求的特定特性进行调节。通过这项研究了解改变明胶-SNP比值对机械和生物学的影响，将为iSTB在特定疾病上的生物医学应用提供启示。所有结果表明，iSTB可以通过未满足临床需求的特定特性进行调节。通过这项研究了解改变明胶-SNP比值对机械和生物学的影响，将为iSTB在特定疾病上的生物医学应用提供启示。

更新日期：2020-02-27

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>