Collagen 1 (COL1) and fibronectin (FN) are extracellular matrix proteins that contribute in cell activity and involve in regulating dental pulp cells (DPCs). The purpose of this study was to investigate the effect of COL1 and FN on the behavior of DPCs. Here, DPCs were grown under three different conditions: COL1 coating, FN coating, and control group without coating. The proliferation and differentiation of DPCs were investigated. DPCs in osteogenic media were able to differentiate into osteoblastic phenotype. The morphological analysis revealed no obvious difference on the shape of cells. Cells had spread well on both coated and noncoated culture plates with slightly more spreading in the coated plates after 24 hr. The MTT analysis did not demonstrate a significant difference at 1 and 3 hr among the groups, but interestingly, the analysis disclosed more cells on the coated plates after longer cultures, which indicated a higher proliferative capacity in response to COL1 and FN. RT‐PCR, Western Blotting and mineralization assays did not reveal significant differences between the coated and noncoated surfaces in relation to osteogenic differential potential. Our data suggested that the surface coating of COL1 and FN were able to promote cellular proliferation and the osteogenic differentiation tendency of DPCs was also observed in vitro.

中文翻译：

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牙髓细胞对胶原蛋白 1 和纤连蛋白修饰的表面的生物学反应。

胶原蛋白 1 (COL1) 和纤连蛋白 (FN) 是细胞外基质蛋白，它们有助于细胞活性并参与调节牙髓细胞 (DPC)。本研究的目的是研究 COL1 和 FN 对 DPC 行为的影响。在这里，DPC 在三种不同的条件下生长：COL1 涂层、FN 涂层和没有涂层的对照组。研究了DPC的增殖和分化。成骨培养基中的 DPC 能够分化为成骨细胞表型。形态学分析显示细胞形态无明显差异。细胞在包被和未包被的培养板上扩散良好，24 小时后在包被板中扩散稍多。MTT 分析未显示各组在 1 小时和 3 小时时有显着差异，但有趣的是，分析显示，经过更长时间的培养后，包被板上的细胞更多，这表明对 COL1 和 FN 的反应具有更高的增殖能力。RT-PCR、Western Blotting 和矿化分析未显示涂层和非涂层表面在成骨差异潜能方面的显着差异。我们的数据表明，COL1 和 FN 的表面涂层能够促进细胞增殖，并且还在体外观察到 DPC 的成骨分化趋势。