Cd is a non-essential metal and highly toxic to plants, animals and humans, even at very low concentrations. Cd has been found in cocoa beans and in their products, as in the case of chocolate. Mn plays an important role in photosynthetic and can interact with Cd and attenuate its toxic effects on plants. The objective of this work was to evaluate the mechanisms of Mn response in the mitigation of Cd toxicity in young plants of the CCN 51 cacao genotype submitted to 0.8 mmol Cd kg-1, 1.6 mmol Mn kg-1 or the combination of 0.4 mmol Cd kg-1 + 0.8 mmol Mn kg-1 soil, together with the control treatment (without addition of Cd and Mn in soil), by means of analysis of changes in the profile of exclusive proteins (EP) and differentially accumulated proteins (DAP). Leaf and root proteins were extracted and quantified from the different treatments, followed by proteomic analysis. About eight DAP and 38 EP were identified in leaves, whereas in roots 43 DAP and 21 EP were identified. Some important proteins induced in the presence of Cd and repressed in the presence of Cd + Mn or vice versa, were ATPases, isoflavone reductase, proteasome and chaperonin. It was concluded that proteins involved in oxidoreduction and defense and stress response processes, in addition to other processes, were induced in the presence of Cd and repressed in the presence of Cd + Mn. This demonstrated that Mn was able to mitigate the toxic effects of Cd on young plants of the CCN 51 cocoa genotype.

中文翻译：

---

通过叶和根的蛋白质组学分析评估了锰对可可幼树中Cd毒性的缓解作用。

Cd是一种非必需金属，即使浓度很低，也对植物，动物和人类有剧毒。在可可豆及其制品中发现了镉，例如巧克力。Mn在光合作用中起重要作用，并且可以与Cd相互作用并减弱其对植物的毒性作用。这项工作的目的是评估CCN 51可可基因型提交0.8 mmol Cd kg-1、1.6 mmol Mn kg-1或0.4 mmol Cd的组合对缓解Mn缓解Cd毒性的机制。 kg-1 + 0.8 mmol Mn kg-1土壤，以及对照处理（在土壤中不添加Cd和Mn），通过分析排他蛋白（EP）和差异累积蛋白（DAP）的特征变化。从不同处理中提取和定量叶和根蛋白，然后进行蛋白质组学分析。在叶片中鉴定出约八种DAP和38 EP，而在根中鉴定出43种DAP和21 EP。在Cd的存在下诱导并在Cd + Mn的存在下受到抑制或反之亦然的一些重要蛋白质是ATPase，异黄酮还原酶，蛋白酶体和伴侣蛋白。结论是，除了其他过程外，在Cd存在下还诱导了参与氧化还原和防御以及应激反应过程的蛋白质，在Cd + Mn存在下被抑制。这表明Mn能够减轻Cd对CCN 51可可基因型年轻植物的毒性作用。在Cd的存在下诱导并在Cd + Mn的存在下受到抑制或反之亦然的一些重要蛋白质是ATPase，异黄酮还原酶，蛋白酶体和伴侣蛋白。结论是，除了其他过程外，在Cd存在下还诱导了参与氧化还原和防御以及应激反应过程的蛋白质，在Cd + Mn存在下被抑制。这表明Mn能够减轻Cd对CCN 51可可基因型年轻植物的毒性作用。在Cd的存在下诱导并在Cd + Mn的存在下受到抑制或反之亦然的一些重要蛋白质是ATPase，异黄酮还原酶，蛋白酶体和伴侣蛋白。结论是，除了其他过程外，在Cd存在下还诱导了参与氧化还原和防御以及应激反应过程的蛋白质，在Cd + Mn存在下被抑制。这表明Mn能够减轻Cd对CCN 51可可基因型年轻植物的毒性作用。