The simultaneous and sequential dual elicitation effect on plumbagin production in Plumbago indica L. root cultures, revealed that combination of chitosan (150 mg L⁻¹) with ʟ-alanine (5 mM) or methyl-β-cyclodextrin (MCD; 2 mM) significantly increased plumbagin production, but in the different treatment manners. The simultaneous treatment using chitosan + ʟ-alanine on a 14-day-old culture enhanced plumbagin production to 14.62 mg g⁻¹ DW, while the sequential additions of MCD to a 12-day-old culture followed by chitosan after 48 h enhanced production of plumbagin to 14.33 mg g⁻¹ DW. The plumbagin productivity from both treatments were up to 1.3- and 8-fold higher than the chitosan treated (10.93 mg g⁻¹ DW) and untreated root cultures (1.76 mg g⁻¹ DW). Moreover, the present studies provided new information on the effect of simultaneous and sequential elicitation on plumbagin-producing P. indica root cultures using chitosan in combinations with MCD or ʟ-alanine.

中文翻译：

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通过壳聚糖与β-丙氨酸和甲基-β-环糊精的同时和顺序双重诱导，提高了黑root根培养中的羽扇豆产量

上白花丹素生产在同时和顺序双重引出效果紫雪花L.根培养物，脱乙酰壳多糖显示（150毫克的L的该组合^-1）与ʟ丙氨酸（5毫摩）或甲基β环糊精（MCD; 2mM）的大大提高了李子的产量，但采用不同的处理方式。在14天龄的培养物中同时使用壳聚糖+β-丙氨酸进行处理，可将plumbagin的产量提高至14.62 mg g ^-1 DW，而向12天龄的培养物中依次添加MCD，然后在48 h后加入壳聚糖，则可提高产量李白蛋白至14.33 mg g ^-1 DW。两种处理的铅皮素生产率分别比脱乙酰壳多糖处理（10.93 mg g ^-1）高1.3到8倍DW）和未经处理的根培养物（1.76 mg g ^-1 DW）。此外，本研究提供了关于同时和顺序激发对使用壳聚糖与MCD或β-丙氨酸结合的铅素产生的P. indica根培养物的影响的新信息。