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Nanoparticle-immersed paper imprinting mass spectrometry imaging reveals uptake and translocation mechanism of pesticides in plants
Nano Research ( IF 9.5 ) Pub Date : 2020-02-28 , DOI: 10.1007/s12274-020-2700-5
Xinzhou Wu , Run Qin , Hanxiang Wu , Guangkai Yao , Yue Zhang , Ping Li , Yizhu Xu , Zhixiang Zhang , Zhibin Yin , Hanhong Xu

Abstract

Design and discovery of carrier-mediated modified pesticides are vital for reducing pesticide dosage and increasing utilization, yet it remains a great challenge due to limited insights into plant translocation mechanisms. Nanostructure/nanoparticle assisted laser desorption/ionization strategy has established itself as a preferential analytical tool for biological tissue analysis, whereas potential applications in plant sciences are hindered with regard to the inability to slice plant leaves and petals. Herein, we report gold nanoparticle (AuNP)-immersed paper imprinting mass spectrometry imaging (MSI) for the spatiotemporal visualization of pesticide translocation in plant leaves. This approach plays a dual role in preserving spatial information and improving ionization efficiency for pesticides regardless of imaging artifacts due to homogenous AuNP deposition. Using this MSI platform, we proposed the elaborate plant translocation mechanism of agrochemicals for the first time, which is currently poorly understood. The dynamic processes of carrier-mediated pesticides can be clearly visualized, including crossing of plasma membranes by transporters, translocation downward in stems through the phloem, diffusion to the xylem and, conversely, accumulation at margins of the treated leaves. Moreover, this AuNP-assisted paper imprinting method could be highly compatible with laser-based MSI instruments, expediting researches across a broad range of fields, especially in nanomaterial development and life sciences.



中文翻译:

纳米浸没纸印记质谱成像揭示了农药在植物体内的吸收和转运机制

摘要

载体介导的改性农药的设计和发现对于降低农药用量和提高利用率至关重要,但由于对植物易位机制的了解有限,仍然是一项巨大的挑战。纳米结构/纳米粒子辅助的激光解吸/电离策略已经确立了其作为生物组织分析的优先分析工具的地位,但是由于无法切片植物的叶子和花瓣而阻碍了其在植物科学中的潜在应用。在这里,我们报告沉金纳米粒子(AuNP)浸没纸印质谱成像(MSI)的农药叶在植物叶片中的时空可视化。这种方法在保留空间信息和提高农药的电离效率方面都起着双重作用,而不管由于均匀的AuNP沉积而导致的成像伪影。使用该MSI平台,我们首次提出了精细的农用化学品植物易位机制,目前尚不清楚。可以清楚地看到载体介导的农药的动态过程,包括转运蛋白穿越质膜,在茎中穿过韧皮部向下移位,向木质部扩散以及相反地在处理过的叶片边缘积累。而且,这种AuNP辅助的纸张压印方法可能与基于激光的MSI仪器高度兼容,从而加速了广泛领域的研究,尤其是在纳米材料开发和生命科学领域。使用该MSI平台,我们首次提出了精细的农用化学品植物易位机制,目前尚不清楚。可以清楚地看到载体介导的农药的动态过程,包括转运蛋白穿越质膜,在茎中穿过韧皮部向下移位,向木质部扩散以及相反地在处理过的叶片边缘积累。此外,这种AuNP辅助的纸张压印方法可能与基于激光的MSI仪器高度兼容,从而加快了广泛领域的研究速度,尤其是在纳米材料开发和生命科学领域。使用该MSI平台,我们首次提出了精细的农用化学品植物易位机制,目前尚不清楚。可以清楚地看到载体介导的农药的动态过程,包括转运蛋白穿越质膜,在茎中穿过韧皮部向下移位,向木质部扩散以及相反地在处理过的叶片边缘积累。而且,这种AuNP辅助的纸张压印方法可能与基于激光的MSI仪器高度兼容,从而加速了广泛领域的研究,尤其是在纳米材料开发和生命科学领域。可以清楚地看到载体介导的农药的动态过程,包括转运蛋白穿越质膜,在茎中穿过韧皮部向下移位,向木质部扩散以及相反地在处理过的叶片边缘积累。而且,这种AuNP辅助的纸张压印方法可能与基于激光的MSI仪器高度兼容,从而加速了广泛领域的研究,尤其是在纳米材料开发和生命科学领域。可以清楚地看到载体介导的农药的动态过程,包括转运蛋白穿越质膜,在茎中穿过韧皮部向下移位,向木质部扩散以及相反地在处理过的叶片边缘积累。而且,这种AuNP辅助的纸张压印方法可能与基于激光的MSI仪器高度兼容,从而加速了广泛领域的研究,尤其是在纳米材料开发和生命科学领域。

更新日期:2020-02-28
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