In this study, the polysaccharide ascophyllan, isolated from Ascophyllum nodosum, was degraded using alginate lyase (EC 4.2.2.3) to prepare low-molecular-weight fragments. LMWAs-L, an extremely low-molecular-weight saccharide (6.71 kDa) with low fucose- and sulphate-contents, was purified by gel filtration chromatography. LMWAs-L exhibited significant macrophage-stimulating activities, similar to ascophyllan, through intracellular signalling pathways. Methylation and GC/MS analyses indicated that the main glycosidic linkages of LMWAs-L were 1,2-linked-GlcpA, followed by T-linked-Glcp, 1,3,6-linked-Galp, 1,3-linked-Fucp, 1,4-linked-Xylp, and 1,4-linked-ManpA at a molar ratio of 3.37: 1.13: 1.11: 1.00: 0.96: 0.61. The proposal repeating structure of LMWAs-L was shown to be →2)-α-D-GlcpA-(1 → 2)-α-D-GlcpA-(1 → 6)-α-D-Galp-(1 → 2)-α-D-GlcpA-(1→ as main chain, branched by T-α-D-Glcp-(1 → 4)-β-D-Xylp-(1 → 3)-α-L-Fucp4S-(1→ at the O-3 of 3,6)-α-D-Galp-(1→ residues. 4-deoxy-L-erythro-hex-4-enuronosyluronate-4-β-D-ManpA-(1→ and →4)-β-D-ManpA_red residues were attached to the ends of main chain as non-reducing end residue and reducing end residue, respectively. Our results suggest that the fragment LMWAs-L contain an essential structural element of ascophyllan that is responsible for the macrophage-stimulating activities.

在这项研究中，使用藻酸盐裂解酶（EC 4.2.2.3）降解了从结节藻中分离出来的多糖类多糖，以制备低分子量片段。LMWAs-L是一种具有极低的岩藻糖和硫酸盐含量的极低分子量糖（6.71 kDa），可通过凝胶过滤色谱法纯化。LMWAs-L通过细胞内信号传导途径表现出显着的巨噬细胞刺激活性，类似于椰油胞苷。甲基化和GC / MS分析表明，LMWAs-L的主要糖苷键是1,2-Glc p A，其次是T -Glc p 1,3,6-6-Gal p 1,3。键联的Fuc p，1,4-键联的Xyl p和1,4-键联的Manp A的摩尔比为3.37：1.13：1.11：1.00：0.96：0.61。的LMWAs-L的建议重复结构被证明是→2）-α-d-GLC p A-（1→2）-α-d-GLC p A-（1→6）-α-d-Gal的p - （1→2）-α-d-GLC p A-（1→作为主链，通过支Ť -α-d-GLC p - （1→4）-β-d-的Xyl p - （1→3 ）-α-L-Fuc的p 4S-（1→在Ô -3 3,6）-α-d-Gal的p - （1个→4残基脱氧-1-赤-己-4- enuronosyluronate- 4-β-d-曼p A-（1→和→4）-β-d-曼p甲_红色残基分别作为非还原性末端残基和还原性末端残基连接至主链末端。我们的结果表明，片段LMWAs-L包含必需的结构性抗坏血酸结构元件，其负责刺激巨噬细胞。