The establishment of a chronic inflammatory state in the adipose tissue contributes to obesity-associated insulin resistance. Hence, disrupting the inflammatory response elicited by obesity remains a relevant target to tackle the modern-world pandemic. We evaluated the anti-inflammatory and insulin-sensitizing effect of Calafate (Berberis microphylla) by producing and characterizing a polyphenol-pure Calafate extract (PPCE). C57BL/6 mice fed with cafeteria diet for 14 weeks were administered PPCE (50 mg/Kg/day) for 4 weeks. PPCE administration rescued glucose tolerance and insulin-elicited AKT phosphorylation in white adipose tissue of diet-induced insulin-resistant mice. Furthermore, the cafeteria diet-induced expression of TNF-alpha and F4/80 was attenuated by PPCE administration, suggesting that PPCE rescues insulin sensibility by ameliorating the obesity-associated inflammatory state. Altogether, our data shows that Calafate represents a natural source of polyphenols with glucose tolerance-improving properties in vivo, suggesting a potential use of PPCE as a complementary tool against insulin resistance.

脂肪组织中慢性炎症状态的建立有助于肥胖相关的胰岛素抵抗。因此，破坏由肥胖引起的炎症反应仍然是解决现代流行病的重要目标。我们评估了Calafate（小ber小叶菊）的抗炎和胰岛素敏感作用），并通过生产和表征纯多酚纯草酸盐提取物（PPCE）。用食堂饮食喂养14周的C57BL / 6小鼠接受PPCE（50 mg / Kg /天）治疗4周。在饮食诱导的胰岛素抵抗小鼠的白色脂肪组织中，PPCE给药可挽救葡萄糖耐量和胰岛素引发的AKT磷酸化。此外，通过PPCE施用，食堂饮食诱导的TNF-α和F4 / 80的表达减弱，表明PPCE通过改善肥胖相关的炎症状态来拯救胰岛素敏感性。总而言之，我们的数据表明，卡拉法特代表多酚的天然来源，具有体内葡萄糖耐量提高的特性，表明PPCE有可能用作抗胰岛素抵抗的补充工具。