The Molecular Anatomy of Mouse Skin during Hair Growth and Rest.,Cell Stem Cell

当前位置： X-MOL 学术 › Cell Stem Cell › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

The Molecular Anatomy of Mouse Skin during Hair Growth and Rest.
Cell Stem Cell ( IF 19.8 ) Pub Date : 2020-02-15 , DOI: 10.1016/j.stem.2020.01.012
Simon Joost ₁ , Karl Annusver ₁ , Tina Jacob ₁ , Xiaoyan Sun ₁ , Tim Dalessandri ₁ , Unnikrishnan Sivan ₁ , Inês Sequeira ₂ , Rickard Sandberg ₃ , Maria Kasper ₁

Affiliation

Skin homeostasis is orchestrated by dozens of cell types that together direct stem cell renewal, lineage commitment, and differentiation. Here, we use single-cell RNA sequencing and single-molecule RNA FISH to provide a systematic molecular atlas of full-thickness skin, determining gene expression profiles and spatial locations that define 56 cell types and states during hair growth and rest. These findings reveal how the outer root sheath (ORS) and inner hair follicle layers coordinate hair production. We found that the ORS is composed of two intermingling but transcriptionally distinct cell types with differing capacities for interactions with stromal cell types. Inner layer cells branch from transcriptionally uncommitted progenitors, and each lineage differentiation passes through an intermediate state. We also provide an online tool to explore this comprehensive skin cell atlas, including epithelial and stromal cells such as fibroblasts, vascular, and immune cells, to spur further discoveries in skin biology.

中文翻译：

小鼠皮肤在毛发生长和休息期间的分子解剖。

皮肤动态平衡由数十种细胞类型精心策划，这些细胞类型共同指导干细胞更新，谱系承诺和分化。在这里，我们使用单细胞RNA测序和单分子RNA FISH来提供全厚度皮肤的系统分子图集，确定基因表达谱和空间位置，以定义毛发生长和休息期间的56种细胞类型和状态。这些发现揭示了外部根鞘（ORS）和内部毛囊层如何协调头发的产生。我们发现ORS由两种相互混合但转录上不同的细胞类型组成，这些细胞类型具有与基质细胞类型相互作用的能力。内层细胞从转录上未提交的祖细胞分支，并且每个谱系分化都通过中间状态。

更新日期：2020-02-28

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11